FastQCFastQC Report
Thu 26 May 2016
SRR1512381_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512381_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2977768
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT99810.3351839364248659No Hit
CTGTAGGACGTGGAATATGGCAAGA89800.30156815440289503No Hit
GTCCTAAAGTGTGTATTTCTCATTT85370.2866912398816832No Hit
GTATCAACGCAGAGTACTTTTTTTT74240.24931425147963174No Hit
CTTTAGGACGTGAAATATGGCGAGG71130.2388701873349435No Hit
GTCCTACAGTGTGCATTTCTCATTT53930.18110880364084778No Hit
GGTATCAACGCAGAGTACTTTTTTT45430.1525639337920214No Hit
TATCAACGCAGAGTACTTTTTTTTT42870.14396689063755136No Hit
CTGAAGGACCTGGAATATGGCGAGA38710.1299966955115375No Hit
CTGTAGGACCTGGAATATGGCGAGA38240.1284183321198965No Hit
GTCCTTCAGTGTGCATTTCTCATTT34510.11589217158623505No Hit
ATTTAGAAATGTCCACTGTAGGACG31720.10652273783585559No Hit
GAATATGGCAAGAAAACTGAAAATC30070.10098167486520106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15250.014.4846131
TAGGACC18250.013.615594
TACGGCG604.1272934E-412.65449916
TCCAACG1950.012.14828318
GGCGAGG21500.011.81550819
AGGACCT34600.011.5125425
TGTAGGA57050.011.4871732
AAATGTC13100.011.1492987
AATGTCC13400.011.1436518
GGACCTG34600.011.128796
GTAGGAC57500.011.0840763
ATACCGT600.005951326311.0648326
TATGCGC600.005978306311.05810910
GTCTTAC3500.010.9881691
GTCCTAA26050.010.90218451
CTGTAGG52200.010.8435161
GAAATGT13800.010.789936
GTCCTAC50200.010.7844331
GTGTAGG6700.010.7442671
ATGTCCA14200.010.65576659
Produced by FastQC (version 0.11.5)