FastQCFastQC Report
Thu 26 May 2016
SRR1512381_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512381_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2977768
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA107480.3609414836884539No Hit
GTCCTACAGTGGACATTTCTAAATT106320.35704594850908467No Hit
GTATCAACGCAGAGTACTTTTTTTT93420.3137249107385129No Hit
GTCCTAAAGTGTGTATTTCTCATTT91610.30764653257070396No Hit
CTTTAGGACGTGAAATATGGCGAGG85520.2871949728790154No Hit
GGTATCAACGCAGAGTACTTTTTTT60440.20297081572506653No Hit
TATCAACGCAGAGTACTTTTTTTTT60100.20182902093111352No Hit
GTCCTACAGTGTGCATTTCTCATTT58430.19622079356081465No Hit
CTGAAGGACCTGGAATATGGCGAGA47340.1589781339580518No Hit
CTGTAGGACCTGGAATATGGCGAGA43780.1470228708213669No Hit
GTCCTTCAGTGTGCATTTCTCATTT36440.12237353615190975No Hit
ATTTAGAAATGTCCACTGTAGGACG35140.11800785017503043No Hit
GAATATGGCAAGAAAACTGAAAATC33540.11263469820348665No Hit
TTTCTAAATTTTCCACCTTTTTCAG32370.10870558082429524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16250.014.2303271
TAGGACC15450.013.0454854
TGGACCG701.0836801E-412.2237555
GTCGAGG1603.6379788E-1211.86844119
CACCTTT12750.011.54341514
GGCGAGG20850.011.47567319
TACGGTG855.2845477E-511.1851345
TATACCG600.005843994711.0919255
ACCGGGC600.005885076211.0814918
CCACCTT12450.011.06074513
GATATAC7400.011.0593271
TGGCGAA2150.011.0404118
AGGACCT30900.011.0150065
ATTGCGA951.3696037E-510.99392417
TGTAGGA56850.010.8866962
TCGAACT1752.0008883E-1110.85114719
TAGGACA14650.010.8375644
TCCAACG2200.010.78949218
GTATCAA38250.010.7476691
GAAATGT13450.010.7348176
Produced by FastQC (version 0.11.5)