FastQCFastQC Report
Thu 26 May 2016
SRR1512380_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512380_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2827401
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT95230.33681108551634525No Hit
CTGTAGGACGTGGAATATGGCAAGA87100.30805676308383567No Hit
GTCCTAAAGTGTGTATTTCTCATTT81060.28669438823852716No Hit
GTATCAACGCAGAGTACTTTTTTTT74400.26313918683625No Hit
CTTTAGGACGTGAAATATGGCGAGG66330.23459707342538255No Hit
GTCCTACAGTGTGCATTTCTCATTT51530.18225218142032207No Hit
GGTATCAACGCAGAGTACTTTTTTT46380.16403757372937194No Hit
TATCAACGCAGAGTACTTTTTTTTT44130.15607973541779183No Hit
CTGAAGGACCTGGAATATGGCGAGA36280.12831572175294553No Hit
CTGTAGGACCTGGAATATGGCGAGA35440.1253447954499556No Hit
GTCCTTCAGTGTGCATTTCTCATTT32460.11480508070839615No Hit
ATTTAGAAATGTCCACTGTAGGACG29220.10334579353972076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC16550.014.1000834
GGTATCA15400.012.6151171
GTCCTAG4250.012.2979491
AATCCCG3150.011.43085519
AGGACCT31700.011.37146955
GGCGAGG20000.011.32331519
TGTAGGA52150.011.3141162
GGACCTG30450.011.2463666
CATTTCG600.005925090511.07137114
CCAACGT1303.3958713E-810.93335819
GTAGGAC52800.010.9233083
CTGTAGG46500.010.9219461
CCGTTCG700.001506447210.84465216
GTCCTAC48850.010.8002791
GTGGTAT5850.010.7887191
GTGTAGG5050.010.7403151
GTCCTAA24850.010.6750931
GACGTGG24750.010.578557
ACCTTTT10200.010.51315115
TGGTATC5700.010.4845612
Produced by FastQC (version 0.11.5)