FastQCFastQC Report
Thu 26 May 2016
SRR1512380_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512380_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2827401
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT102280.36174564555929634No Hit
CTGTAGGACGTGGAATATGGCAAGA98900.34979120400678926No Hit
GTATCAACGCAGAGTACTTTTTTTT95540.33790749879482956No Hit
GTCCTAAAGTGTGTATTTCTCATTT87080.30798602674328823No Hit
CTTTAGGACGTGAAATATGGCGAGG79020.2794792815026945No Hit
GGTATCAACGCAGAGTACTTTTTTT62340.2204851734861804No Hit
TATCAACGCAGAGTACTTTTTTTTT62330.22044980531590674No Hit
GTCCTACAGTGTGCATTTCTCATTT52420.18539994857468042No Hit
CTGAAGGACCTGGAATATGGCGAGA45860.1621984288751401No Hit
CTGTAGGACCTGGAATATGGCGAGA42420.1500317783009909No Hit
GTCCTTCAGTGTGCATTTCTCATTT34290.12127745586848132No Hit
ATTTAGAAATGTCCACTGTAGGACG33770.11943831101424948No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30470.10776681482393194No Hit
TTTCTAAATTTTCCACCTTTTTCAG30320.1072362922698266No Hit
GAATATGGCAAGAAAACTGAAAATC30040.1062459835021633No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAG350.002178743716.276818
GTGCGTC456.771969E-414.775711
GGTATCA17250.014.0641471
TAGGACC17050.013.2153454
TTGGCGA802.8844195E-511.86850217
GGCGAGG21650.011.62181219
CACCTTT11950.011.60095814
AGGACCT32550.011.4203745
CTAGGAC4450.011.3230323
GGACCTG31050.011.3190596
TCCAACG2350.011.31295418
CCACCTT12100.011.2235113
GTCCTAT4300.011.062771
TGTAGGA55050.010.9663792
TTAGGAC30000.010.9648263
AGGACGT51800.010.9388355
TAGAAAT12550.010.9087084
GGACGTG51100.010.8558086
CCAACGA1752.0008883E-1110.85120119
TTCCACC12050.010.79931211
Produced by FastQC (version 0.11.5)