FastQCFastQC Report
Thu 26 May 2016
SRR1512379_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512379_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2657648
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT79990.300980415766121No Hit
GTATCAACGCAGAGTACTTTTTTTT69720.26233722449323615No Hit
CTGTAGGACGTGGAATATGGCAAGA66060.24856564902500256No Hit
GTCCTAAAGTGTGTATTTCTCATTT64160.24141647050324194No Hit
CTTTAGGACGTGAAATATGGCGAGG50220.18896407650674582No Hit
GTCCTACAGTGTGCATTTCTCATTT46600.175343010060023No Hit
GGTATCAACGCAGAGTACTTTTTTT42260.1590127812261067No Hit
TATCAACGCAGAGTACTTTTTTTTT38680.14554222380089463No Hit
CTGTAGGACCTGGAATATGGCGAGA31060.11687025520309686No Hit
CTGAAGGACCTGGAATATGGCGAGA26690.10042714460304751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14100.014.0600454
GGTATCA15350.013.3020131
TAGCACG550.003104771712.0724364
GTCTAAT1653.6379788E-1211.9564291
CGGTAGG600.0044408211.5080621
GGCGAGG16600.011.30246319
TGTAGGA42200.011.2838952
AGGACCT26100.011.2662855
GGACCTG24750.011.1909566
GTAGGAC41050.011.0454263
GTCCTAC39550.010.8990931
CTGTAGG39150.010.7332991
TATGTCG1951.8189894E-1210.70400316
ATGTCGA2150.010.59105417
TTAGGAC22350.010.5679223
GGACGTG37450.010.5366136
TGGCGAG39550.010.49029318
CTAGGAC3900.010.4585533
AGGACGT38550.010.4574095
GACGTGG18800.010.293157
Produced by FastQC (version 0.11.5)