FastQCFastQC Report
Thu 26 May 2016
SRR1512379_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512379_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2657648
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87760.3302167931945841No Hit
GTCCTACAGTGGACATTTCTAAATT81040.3049312775807782No Hit
CTGTAGGACGTGGAATATGGCAAGA78010.2935302192013389No Hit
GTCCTAAAGTGTGTATTTCTCATTT66380.24976972119708854No Hit
CTTTAGGACGTGAAATATGGCGAGG59870.22527437794621408No Hit
GGTATCAACGCAGAGTACTTTTTTT55930.2104492393274053No Hit
TATCAACGCAGAGTACTTTTTTTTT54940.20672414104501421No Hit
GTCCTACAGTGTGCATTTCTCATTT47140.1773748818504181No Hit
CTGTAGGACCTGGAATATGGCGAGA37030.1393337266635762No Hit
CTGAAGGACCTGGAATATGGCGAGA31030.11675737343696381No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27910.10501766975912535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTCG350.002158662816.2993285
TTGGCGA500.001506138813.29283517
TAGGACC13550.012.9813224
GGTATCA16500.012.6298631
GGCGAGG19300.012.2990719
TCCAACG802.8840122E-511.86860418
GGACCGT752.0780046E-411.3981486
TGGCGAG42550.011.15732418
GTGGTAT6450.011.064711
AGGACCT26350.011.0414815
GGACCTG25400.011.0316736
TTAGGAC25150.010.9256273
CTAGGAC4200.010.86621953
ACCGTCT700.001494498610.8549718
TTAGACC1502.1063897E-910.7756664
GACGTGA23100.010.6905017
CAACCGT803.7782392E-410.6857646
TGTAGGA44050.010.6848962
AGGACGT43550.010.6759665
GGACGTG42800.010.56370456
Produced by FastQC (version 0.11.5)