FastQCFastQC Report
Thu 26 May 2016
SRR1512378_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512378_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025258
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66280.3272669457422215No Hit
GTCCTACAGTGGACATTTCTAAATT62460.30840515134368063No Hit
GTCCTAAAGTGTGTATTTCTCATTT60070.2966041857383109No Hit
CTGTAGGACGTGGAATATGGCAAGA57800.28539573723446593No Hit
CTTTAGGACGTGAAATATGGCGAGG50110.24742526631174894No Hit
GGTATCAACGCAGAGTACTTTTTTT42270.20871414901212584No Hit
TATCAACGCAGAGTACTTTTTTTTT38050.18787729760850222No Hit
GTCCTACAGTGTGCATTTCTCATTT32550.1607202637886136No Hit
CTGAAGGACCTGGAATATGGCGAGA27270.1346495113215205No Hit
GTCCTTCAGTGTGCATTTCTCATTT24320.1200834659090348No Hit
ATTTAGAAATGTCCACTGTAGGACG23630.11667649257526694No Hit
GAATATGGCAAGAAAACTGAAAATC21020.10378924561710162No Hit
CTGTAGGACCTGGAATATGGCGAGA20900.10319672851557679No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC457.009918E-414.7081998
GGTATCA11850.014.3972651
TAGGACC10550.013.8451894
TCCAACG1102.8685463E-912.917212518
GGCGAGG13700.012.24169119
TACCGTA550.003105302312.0719317
GATCTAT904.920699E-612.0532021
GTCCTAC31550.011.2526651
CACCTTT8200.011.22323114
AGGACCT21700.011.1023625
TGTAGGA34600.010.9105932
TCCTACA35550.010.7791172
CCACCTT8650.010.74557813
GTTTAGG2300.010.7192511
GGACCTG21800.010.7033586
TTGGACG803.8277346E-410.6704614
AAATGTC8450.010.663717
AATGTCC8450.010.6301868
TGTCCAC8950.010.5931310
TGGCGAG34400.010.5740918
Produced by FastQC (version 0.11.5)