FastQCFastQC Report
Thu 26 May 2016
SRR1512378_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512378_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025258
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79730.3936782375381309No Hit
CTGTAGGACGTGGAATATGGCAAGA66940.3305257898006081No Hit
GTCCTACAGTGGACATTTCTAAATT66250.32711881646684027No Hit
GTCCTAAAGTGTGTATTTCTCATTT63470.3133921702815148No Hit
CTTTAGGACGTGAAATATGGCGAGG59190.2922590603271287No Hit
GGTATCAACGCAGAGTACTTTTTTT54700.2700890454450741No Hit
TATCAACGCAGAGTACTTTTTTTTT52850.2609544067965662No Hit
CTGAAGGACCTGGAATATGGCGAGA34020.16797859828229292No Hit
GTCCTACAGTGTGCATTTCTCATTT33650.16615167055259134No Hit
ATTTAGAAATGTCCACTGTAGGACG27050.13356322996872497No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25880.12778618822885776No Hit
CTGTAGGACCTGGAATATGGCGAGA24880.12284854571615073No Hit
TTTCTAAATTTTCCACCTTTTTCAG24770.12230540503975296No Hit
GTCCTTCAGTGTGCATTTCTCATTT24210.11954032523263704No Hit
GAATATGGCAAGAAAACTGAAAATC23640.11672586900039403No Hit
GGAATATGGCGAGAAAACTGAAAAT20670.10206107073765416No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20410.10077728368435035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14650.012.8582151
TAGGACC11150.012.3630054
AATGTCC8600.011.9289458
ACCGTGC658.0325303E-411.6910468
GGCGAGG15050.011.54540119
TAGAAAT10050.011.5405024
AAATGTC9000.011.5043147
GGACCTG21050.011.100946
TTTAGAA10900.010.9902392
AGGACCT21500.010.9664385
GAAATGT9750.010.9116436
ATGTCCA9450.010.8559719
ACCTTTT9300.010.72014715
CACCTTT9350.010.66282114
TGTCCAC10700.010.65305510
ACGGTAC1252.2190943E-710.64753153
TTAGGAC22000.010.6302463
GTATCAA30100.010.4619981
TTAGACA2100.010.4121264
GCCCTAT1201.4959987E-610.3065951
Produced by FastQC (version 0.11.5)