FastQCFastQC Report
Thu 26 May 2016
SRR1512377_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512377_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047162
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69360.33881050937834917No Hit
GTCCTACAGTGGACATTTCTAAATT50860.24844150096572717No Hit
CTGTAGGACGTGGAATATGGCAAGA48550.23715758694231331No Hit
GTCCTAAAGTGTGTATTTCTCATTT48170.2353013586614054No Hit
GGTATCAACGCAGAGTACTTTTTTT45680.22313817861019303No Hit
CTTTAGGACGTGAAATATGGCGAGG41690.20364778166065997No Hit
TATCAACGCAGAGTACTTTTTTTTT40630.19846988171918004No Hit
GTCCTACAGTGTGCATTTCTCATTT27590.1347719428164454No Hit
CTGAAGGACCTGGAATATGGCGAGA21260.10385108750553204No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTGC400.00545667114.1794088
GGTATCA13300.013.8675411
GACTCGA500.001523150613.27278811
TCTATAC1750.012.4653723
GTATTAA2100.012.2113481
TAGAACG550.003107282612.0709134
TAGGACC7750.011.74828154
AGGACCG907.6003107E-611.5921955
AGAACCG855.4136624E-511.1582615
GCGTTTC600.005943029711.06663613
GTAATGC951.3854671E-510.9820793
AATGCCG2000.010.89542318
ACCTTTT7700.010.8442515
CCTATAC1408.569259E-910.8394553
GGCGAGG13050.010.67453619
GTCCTAC25850.010.5688751
CCGAATG1355.920083E-810.52697918
GTATTAG1501.36496965E-810.5205461
GTGTAAG2350.010.4925651
GACGTGA16550.010.3730557
Produced by FastQC (version 0.11.5)