FastQCFastQC Report
Thu 26 May 2016
SRR1512377_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512377_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047162
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT89380.43660443091460277No Hit
GGTATCAACGCAGAGTACTTTTTTT61680.30129515885894714No Hit
CTGTAGGACGTGGAATATGGCAAGA57570.28121858455754845No Hit
TATCAACGCAGAGTACTTTTTTTTT57350.2801439260791281No Hit
GTCCTACAGTGGACATTTCTAAATT54520.2663199101976297No Hit
GTCCTAAAGTGTGTATTTCTCATTT51610.2521051094148875No Hit
CTTTAGGACGTGAAATATGGCGAGG48270.2357898397879601No Hit
GTCCTACAGTGTGCATTTCTCATTT29960.1463489455157921No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28790.14063371633510197No Hit
CTGAAGGACCTGGAATATGGCGAGA25960.12680970045360357No Hit
ATTTAGAAATGTCCACTGTAGGACG22250.10868705065842373No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22110.10800317708124711No Hit
GTCCTTCAGTGTGCATTTCTCATTT20800.10160407432338037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15650.014.1721171
TAGGACC9550.012.1455774
GTTCTAA1701.8189894E-1211.7588151
CCGGTTT907.486371E-611.607944512
CCTAGAC1406.712071E-1011.5446993
CTACACT3250.011.4088784
ATTAGAC951.3489755E-511.0085683
GCGCCAC951.3670044E-510.99538913
GGCGAGG13050.010.91256319
TGTAGGA31350.010.5847712
CGTGCGC909.532069E-510.553966510
GTATCAA33550.010.4411541
GGACCTG17950.010.424876
GTCCTAA14400.010.3783981
TTAGACT1659.1677066E-1010.3717094
GTGCGCC1106.061484E-610.3615711
TTAGGAC18050.010.2711523
AGGACCT19200.010.2504155
TAGAAAT8350.010.1336354
CTGTAGG31100.010.100591
Produced by FastQC (version 0.11.5)