FastQCFastQC Report
Thu 26 May 2016
SRR1512376_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512376_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978266
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68420.34585844370777236No Hit
GTCCTACAGTGGACATTTCTAAATT58060.2934893487528977No Hit
GTCCTAAAGTGTGTATTTCTCATTT52780.26679930808091534No Hit
CTGTAGGACGTGGAATATGGCAAGA52780.26679930808091534No Hit
CTTTAGGACGTGAAATATGGCGAGG44560.2252477674893063No Hit
GGTATCAACGCAGAGTACTTTTTTT43940.22211370968312655No Hit
TATCAACGCAGAGTACTTTTTTTTT39700.20068079823441337No Hit
GTCCTACAGTGTGCATTTCTCATTT28780.14548094139008608No Hit
CTGAAGGACCTGGAATATGGCGAGA23870.12066122553792058No Hit
GTCCTTCAGTGTGCATTTCTCATTT21930.11085465756374521No Hit
CTGTAGGACCTGGAATATGGCGAGA20680.10453599263193118No Hit
ATTTAGAAATGTCCACTGTAGGACG20580.10403049943738607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13150.014.0245261
AAGCCGT500.001516777713.280146
GCGTGCG905.706406E-712.610569
TAGGACC8850.012.5405674
TAATACG550.003103482312.0728534
TGCGCCA802.9296767E-511.85003712
GTATTAA2700.011.3232291
AGTCGTC3050.011.1580728
AAGTCGT3150.011.1420677
GTCCTAT2600.011.000111
GCGCCAC951.3829231E-510.98385213
TAAGACT1752.1827873E-1110.840934
GTCCTAC27650.010.8073651
GGCGAGG13700.010.78902119
AAATGTC8150.010.70791157
TCTAGGA3650.010.6552972
AGGACCT17650.010.6413095
GGACGTG33300.010.3973646
GAAATGT8500.010.3785966
GTCCTAA16250.010.3780351
Produced by FastQC (version 0.11.5)