FastQCFastQC Report
Thu 26 May 2016
SRR1512376_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512376_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978266
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83470.42193516948681326No Hit
GTCCTACAGTGGACATTTCTAAATT61180.3092607364227056No Hit
CTGTAGGACGTGGAATATGGCAAGA60910.30789590479743373No Hit
GGTATCAACGCAGAGTACTTTTTTT57490.29060803754399056No Hit
GTCCTAAAGTGTGTATTTCTCATTT55670.28140806140326935No Hit
TATCAACGCAGAGTACTTTTTTTTT53230.2690740274563683No Hit
CTTTAGGACGTGAAATATGGCGAGG52890.26735535059491494No Hit
GTCCTACAGTGTGCATTTCTCATTT32180.1626677100046202No Hit
CTGAAGGACCTGGAATATGGCGAGA29540.149322689668629No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26530.13410734451282083No Hit
GTCCTTCAGTGTGCATTTCTCATTT23780.12020628166282997No Hit
TTTCTAAATTTTCCACCTTTTTCAG23340.11798211160683145No Hit
CTGTAGGACCTGGAATATGGCGAGA23330.11793156228737693No Hit
ATTTAGAAATGTCCACTGTAGGACG23170.11712277317610473No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20600.1041315980762951No Hit
GAATATGGCAAGAAAACTGAAAATC20490.10357555556229547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1850.014.88464118
TAGGACC9550.013.4390864
GGTATCA16900.012.9488831
TCGGTAA604.040171E-412.68615151
AGGACCG604.0678796E-412.6758785
CCAACGA1207.4578566E-1012.66049919
CCAACGT1001.4523721E-712.34398719
AATCGCC550.003080168712.08502218
AGCTGCG802.8719356E-511.873127
ACACCGA658.0340845E-411.6907516
GGCGAGG13000.011.32140719
TGGTATC5200.011.1526172
GTGGTAT5800.010.8269741
GCATTAT1502.0791049E-910.7832291
TGCCGGT1605.456968E-1010.68553810
TGTAGGA30750.010.4810532
TATGTCG1553.6925485E-910.41428116
TAGGACT3500.010.3217874
GTGTAAG3050.010.29451
GTCCTAT1855.820766E-1110.286071
Produced by FastQC (version 0.11.5)