FastQCFastQC Report
Thu 26 May 2016
SRR1512375_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512375_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2134088
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68450.32074591113393636No Hit
GGTATCAACGCAGAGTACTTTTTTT48080.22529530178699284No Hit
GTCCTACAGTGGACATTTCTAAATT46350.2171887944639584No Hit
GTCCTAAAGTGTGTATTTCTCATTT45130.21147206675638494No Hit
TATCAACGCAGAGTACTTTTTTTTT43950.20594277274414174No Hit
CTGTAGGACGTGGAATATGGCAAGA43030.2016317977515454No Hit
CTTTAGGACGTGAAATATGGCGAGG40000.1874336953302769No Hit
GTCCTACAGTGTGCATTTCTCATTT24040.11264765089349642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13800.014.2235581
TAGGACC9350.012.679314
GGCGAGG14350.012.47981319
CAGCGCA701.1380417E-412.1594799
TCCAACG1201.0433723E-811.84259218
GTATTAG2050.011.5475981
TGTCGAG1303.401874E-810.93162518
TTAGGAC19350.010.8812673
ATGTCGA1408.4983185E-910.8453917
CCAACGA1053.5768844E-610.82904419
ACCGTGC700.001555501810.8028398
TGGCGAG28600.010.73286718
TCGAACT1159.119667E-710.71133719
CACCTTT8000.010.55755814
AGGACCT17700.010.5565235
GTGTAGG3700.010.3967391
GACGTGA17000.010.3777277
CCTCGAA1106.1260653E-610.35241817
CTCGAAC1106.247834E-610.33535418
GTCCTAC24400.010.3082431
Produced by FastQC (version 0.11.5)