FastQCFastQC Report
Thu 26 May 2016
SRR1512375_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512375_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2134088
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88260.41357244874625604No Hit
GGTATCAACGCAGAGTACTTTTTTT63720.2985818766611311No Hit
TATCAACGCAGAGTACTTTTTTTTT61230.2869141291268214No Hit
CTGTAGGACGTGGAATATGGCAAGA49650.2326520743287062No Hit
GTCCTACAGTGGACATTTCTAAATT48790.2286222498791053No Hit
GTCCTAAAGTGTGTATTTCTCATTT47770.22384269064818324No Hit
CTTTAGGACGTGAAATATGGCGAGG47270.22149976945655472No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28120.13176588781718465No Hit
GTCCTACAGTGTGCATTTCTCATTT27780.1301727014068773No Hit
CTGAAGGACCTGGAATATGGCGAGA24160.11320995197948726No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23210.1087584017153932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15550.014.6841591
ACTGCGC500.001500327413.29945858
TAGGACC8550.012.4529884
GGCGAGG13100.012.103119
TGGTATC4500.011.8306172
GTGGTAT5150.011.0844021
ACCGTGA600.005878655711.0828828
GGTCTAG700.001473437810.873271
CACCTTT8700.010.69519714
AGGACCT17150.010.5320065
GTCCTAA15250.010.2939571
TGGCGAG27750.010.16119918
TTAGGAC19300.010.1468583
TCGAAAC750.002662583510.12674816
GGACCTG15950.010.12521656
ACCTTTT9850.010.02487815
GGACGTG29050.010.0067126
CCACCTT9800.09.98218213
AGGACGT29450.09.9745865
CCCGTTT1054.1122308E-59.95104212
Produced by FastQC (version 0.11.5)