FastQCFastQC Report
Thu 26 May 2016
SRR1512374_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512374_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2600939
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT95280.36632923724854755No Hit
CTGTAGGACGTGGAATATGGCAAGA86960.3343407899993041No Hit
GTATCAACGCAGAGTACTTTTTTTT78740.3027368192794987No Hit
GTCCTAAAGTGTGTATTTCTCATTT78470.301698732650016No Hit
CTTTAGGACGTGAAATATGGCGAGG70000.2691335706066155No Hit
GTCCTACAGTGTGCATTTCTCATTT56850.21857490698551563No Hit
GGTATCAACGCAGAGTACTTTTTTT49360.18977761493060777No Hit
TATCAACGCAGAGTACTTTTTTTTT44800.17224548518823393No Hit
CTGTAGGACCTGGAATATGGCGAGA38910.14959981760433444No Hit
CTGAAGGACCTGGAATATGGCGAGA35540.13664295856227307No Hit
GTCCTTCAGTGTGCATTTCTCATTT31330.12045649667293236No Hit
GAATATGGCAAGAAAACTGAAAATC28610.10999873507221815No Hit
ATTTAGAAATGTCCACTGTAGGACG28240.10857617191329746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC16250.014.6494184
GGTATCA14650.014.4066441
TCCAACG2300.013.59223918
CCGGTTT1302.0372681E-1012.39951912
GTGTAGG5750.012.3495561
GCCGGTT1353.8380676E-1011.93912311
AGGACCT31200.011.8554615
ATCCCGT1451.0186341E-1011.76937610
TGTAGGA55600.011.6848782
GGACCGT658.136907E-411.6732946
GGACCTG30400.011.5436986
CGGTTTC1501.8189894E-1011.38322313
CTGTAGG50250.011.2658121
GTAGGAC55500.011.2103533
GTCCTAG4400.011.2073771
GAAATGT13100.011.0773776
TCGTTCA600.006072564511.0348019
GGCGAGG21500.010.92954319
TATGTCG2350.010.90178216
AAATGTC12900.010.8815017
Produced by FastQC (version 0.11.5)