FastQCFastQC Report
Thu 26 May 2016
SRR1512374_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512374_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2600939
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA101100.3887057712618404No Hit
GTATCAACGCAGAGTACTTTTTTTT100800.38755234167352637No Hit
GTCCTACAGTGGACATTTCTAAATT100420.3860913308616619No Hit
GTCCTAAAGTGTGTATTTCTCATTT84550.32507490563984776No Hit
CTTTAGGACGTGAAATATGGCGAGG80630.3100034256858773No Hit
GGTATCAACGCAGAGTACTTTTTTT65540.25198591739367976No Hit
TATCAACGCAGAGTACTTTTTTTTT63960.245911188228559No Hit
GTCCTACAGTGTGCATTTCTCATTT62170.2390290583516184No Hit
CTGTAGGACCTGGAATATGGCGAGA46200.17762815660036627No Hit
CTGAAGGACCTGGAATATGGCGAGA42130.16197996185223876No Hit
GTCCTTCAGTGTGCATTTCTCATTT33500.1287996373617374No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32060.1232631753378299No Hit
ATTTAGAAATGTCCACTGTAGGACG31710.12191750748479684No Hit
GAATATGGCAAGAAAACTGAAAATC30680.1179573992315852No Hit
TTTCTAAATTTTCCACCTTTTTCAG30300.11649638841972072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC16500.013.7718154
GGTATCA15950.013.4201811
ATGCCGT658.037861E-411.6903556
GGACCTG29450.011.6754696
AGGACCT30250.011.6297485
TGTAGGA54200.011.4546762
CTGTAGG53400.011.3305861
GGCGAGG21150.010.99908319
GTAGGAC54600.010.9008153
GACGTGG26150.010.8605187
CGCCCTA1855.456968E-1210.77825216
GGACGTG50750.010.7056016
GCGTTGA803.7236346E-410.7025931
GTCCTAC51800.010.6153571
AGGACGT51600.010.6136995
GTATCAA36500.010.6081111
CGGTTTC2150.010.600636513
ATACGGC909.5638716E-510.55074915
TTTAGAA13300.010.5083512
AAGTCGT3350.010.4907857
Produced by FastQC (version 0.11.5)