FastQCFastQC Report
Thu 26 May 2016
SRR1512373_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512373_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2117108
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT66310.3132102849736528No Hit
CTGTAGGACGTGGAATATGGCAAGA63420.2995595878906508No Hit
GTATCAACGCAGAGTACTTTTTTTT62960.29738681257640137No Hit
GTCCTAAAGTGTGTATTTCTCATTT56700.26781817460422425No Hit
CTTTAGGACGTGAAATATGGCGAGG47740.22549629022232215No Hit
GGTATCAACGCAGAGTACTTTTTTT40670.19210167832722752No Hit
GTCCTACAGTGTGCATTTCTCATTT38640.18251312639695283No Hit
TATCAACGCAGAGTACTTTTTTTTT36610.17292457446667814No Hit
CTGTAGGACCTGGAATATGGCGAGA26760.12639884219416297No Hit
CTGAAGGACCTGGAATATGGCGAGA26190.12370649017433215No Hit
GTCCTTCAGTGTGCATTTCTCATTT22980.10854429722054802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG350.001686122616.912651
GGTATCA13200.014.2753851
TCGCTCG400.00532195914.23297715
TAGGACC12000.013.3561984
TAAGACG604.1528465E-412.6449224
ATTAGAC1457.2759576E-1212.4269063
CGTTTCC1302.0190782E-1012.40823614
ACGGCGA701.1030081E-412.200273517
TACACCG701.1088954E-412.1933185
AACCGTG550.003108256712.0704397
GTGCGTT802.9268716E-511.85123611
GTCTATA2150.011.4717561
CCCCGTA1251.8653736E-811.38638115
GCGTTTC1001.9640174E-611.38367613
CCCGTTT1501.8189894E-1011.37853712
ATCCCGT1251.8897481E-811.37637610
GTCCTAA15900.011.3548761
GCGTGCA752.1600249E-411.3504999
GGCGAGG14600.011.29309219
TCCAACG1950.011.17421318
Produced by FastQC (version 0.11.5)