FastQCFastQC Report
Thu 26 May 2016
SRR1512373_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512373_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2117108
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78280.36974967739010006No Hit
CTGTAGGACGTGGAATATGGCAAGA72840.34405424758680236No Hit
GTCCTACAGTGGACATTTCTAAATT69660.32903375737090407No Hit
GTCCTAAAGTGTGTATTTCTCATTT61010.2881761346138223No Hit
CTTTAGGACGTGAAATATGGCGAGG56860.2685739225396154No Hit
GGTATCAACGCAGAGTACTTTTTTT53560.25298662137217376No Hit
TATCAACGCAGAGTACTTTTTTTTT51960.24542914201826266No Hit
GTCCTACAGTGTGCATTTCTCATTT41690.19691957141534586No Hit
CTGAAGGACCTGGAATATGGCGAGA31360.14812659533665737No Hit
CTGTAGGACCTGGAATATGGCGAGA30730.14515083784105487No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25930.1224783997793216No Hit
GTCCTTCAGTGTGCATTTCTCATTT24520.11581837109868745No Hit
ATTTAGAAATGTCCACTGTAGGACG23750.11218133415961774No Hit
TTTCTAAATTTTCCACCTTTTTCAG21990.10386810687031554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.013.2445021
TAGGACC10500.012.76703554
TTGGCGA604.110452E-412.6603217
TATTGCG751.4868223E-512.6603216
GGCGAGG15000.011.837399519
CGCTCGC658.040188E-411.68976410
CACCTTT7800.011.32178414
AATGTCC7950.011.2305258
CCAACGA1105.0031304E-711.22164719
TTGCGAG855.3519856E-511.17087118
CTGTTCG600.005885341711.0811839
GTGCGCC951.3640143E-510.99760611
TGTAGGA36500.010.9399842
ACCTTTT7900.010.93807215
GGACCTG21100.010.8936216
TTAGGAC19850.010.8245083
AGGACCT22000.010.8038255
TAGGACA8600.010.7234254
TGGCGAG33450.010.70166118
CCACCTT8400.010.62738313
Produced by FastQC (version 0.11.5)