FastQCFastQC Report
Thu 26 May 2016
SRR1512372_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512372_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898736
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT94130.4957508574125102No Hit
GGTATCAACGCAGAGTACTTTTTTT59620.31399836522823604No Hit
TATCAACGCAGAGTACTTTTTTTTT52880.27850106597231No Hit
GTACTGGTTCACTATCGGTCAGTCA36820.19391848050492538No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26340.13872386682508786No Hit
GTCCTACAGTGGACATTTCTAAATT23310.12276588214475315No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22920.12071188411659124No Hit
CTGTAGGACGTGGAATATGGCAAGA22280.11734122068576147No Hit
GTCCTAAAGTGTGTATTTCTCATTT20410.10749256347380573No Hit
GGATACCACGTGTCCCGCCCTACTC19980.10522789898121697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15500.014.5686941
TAGGACC4550.011.2572284
AGGGCCG600.005944767511.06613355
GTACTGG9900.010.8569571
TGGTATC4550.010.8400062
GTATTAC1654.8567017E-1010.7573531
GACGTGA7300.010.5250177
TTAGGAC8800.010.455343
GTATCAA34600.010.4024011
GTATTAG951.1279503E-410.3799011
TACTGGT9200.010.309792
GTAGGAC14000.010.2982793
TGTAGGA15400.010.2856882
ACCGGGG2800.010.16708615
TAGGACG15500.010.0360154
TAGGACA4750.09.9844824
TGGCGAG13000.09.984364518
GTCCTAC12050.09.9836571
CCGGGGG3050.09.95543816
GTCCTAG1709.047653E-99.8609071
Produced by FastQC (version 0.11.5)