FastQCFastQC Report
Thu 26 May 2016
SRR1512372_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512372_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898736
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT118700.6251527331867094No Hit
GGTATCAACGCAGAGTACTTTTTTT79980.4212275956215082No Hit
TATCAACGCAGAGTACTTTTTTTTT76470.4027416133680512No Hit
GTACTGGTTCACTATCGGTCAGTCA38040.20034380766994464No Hit
ACGCAGAGTACTTTTTTTTTTTTTT36610.19281248156668435No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26330.13867120020898113No Hit
GTCCTACAGTGGACATTTCTAAATT25500.13429987107212377No Hit
CTGTAGGACGTGGAATATGGCAAGA25200.13271987258892232No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22860.12039588441995096No Hit
GTCCTAAAGTGTGTATTTCTCATTT22840.12029055118773753No Hit
GGATACCACGTGTCCCGCCCTACTC22600.11902655240117636No Hit
ATCATTAACTGAATCCATAGGTTAA20750.10928322842143404No Hit
CTTTAGGACGTGAAATATGGCGAGG20190.106333897919458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19000.014.5719461
CATTTCG602.579621E-514.24367914
TTTCGCA551.9683434E-413.81168916
TAACCTA1251.3969839E-912.1674245
GTAAGAC1104.9307346E-711.2341283
ACGAAAT1053.4744226E-610.855185512
CGAAATC1053.4788118E-610.85404213
GTATCAA42650.010.6631871
GTGGTAT5150.010.3456781
GTATAGG1303.7631435E-710.2462011
TAGGACA5400.010.2099334
CTGCGTC750.002650258410.1323079
GGCGAGG6400.010.08900619
AACCATC1803.947207E-1010.0267637
TCCTATA2201.8189894E-129.9381452
GTAGGAC13400.09.9314333
TGGTATC5500.09.6788892
CCCCGTA2650.09.67469715
CCCGTAC2650.09.67469716
TAGGACG14350.09.6714074
Produced by FastQC (version 0.11.5)