##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512372_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1898736 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.86545154249985 34.0 31.0 34.0 30.0 34.0 2 32.01773390297545 34.0 31.0 34.0 30.0 34.0 3 32.13466801071871 34.0 31.0 34.0 30.0 34.0 4 35.58803751548398 37.0 35.0 37.0 33.0 37.0 5 35.38889661332592 37.0 35.0 37.0 33.0 37.0 6 35.42253056770399 37.0 35.0 37.0 33.0 37.0 7 35.33288882709339 37.0 35.0 37.0 33.0 37.0 8 35.29734465454913 37.0 35.0 37.0 33.0 37.0 9 36.923733999882025 39.0 37.0 39.0 33.0 39.0 10 36.8656063823512 39.0 37.0 39.0 33.0 39.0 11 36.90864922769674 39.0 37.0 39.0 33.0 39.0 12 36.82523636777309 39.0 37.0 39.0 33.0 39.0 13 36.80148425057512 39.0 37.0 39.0 33.0 39.0 14 38.11995927817242 40.0 38.0 41.0 33.0 41.0 15 38.091956438388486 40.0 38.0 41.0 33.0 41.0 16 38.04427313749779 40.0 38.0 41.0 33.0 41.0 17 38.046713181822014 40.0 38.0 41.0 33.0 41.0 18 37.99928478735327 40.0 38.0 41.0 33.0 41.0 19 38.02101397982658 40.0 38.0 41.0 33.0 41.0 20 37.999903620092525 40.0 38.0 41.0 33.0 41.0 21 37.95779824051369 40.0 38.0 41.0 33.0 41.0 22 37.895539453615456 40.0 38.0 41.0 33.0 41.0 23 37.82123528494746 40.0 38.0 41.0 33.0 41.0 24 37.742331740694866 40.0 38.0 41.0 33.0 41.0 25 37.67231568791027 40.0 38.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35671180358591315 1101 2 0.3170492694409788 1101 3 0.38824177498060664 1101 4 0.2582163270172799 1101 5 0.22599356058594822 1101 6 0.34606719612702364 1101 7 0.4279087371269483 1101 8 0.43250959200072714 1101 9 0.6261825511364236 1101 10 0.5448044215312393 1101 11 0.4564375311986453 1101 12 0.5566430543550283 1101 13 0.6728040051961983 1101 14 0.7488105306426931 1101 15 0.8252497715033655 1101 16 0.7609684696306189 1101 17 0.6810535890435148 1101 18 0.7207537975761227 1101 19 0.6292460896817502 1101 20 0.7548335104024915 1101 21 0.8296406247960917 1101 22 0.8339455279345955 1101 23 0.7522300038190863 1101 24 0.8079628130789729 1101 25 0.774942184199638 1102 1 0.4067687743221349 1102 2 0.32274747119635805 1102 3 0.3472132557684944 1102 4 0.30578519956076633 1102 5 0.20854584956860123 1102 6 0.2952380180850156 1102 7 0.3049828103653667 1102 8 0.3361912553205997 1102 9 0.36972962814280663 1102 10 0.4213843723446189 1102 11 0.3809016678381596 1102 12 0.42906583362444906 1102 13 0.45427272205728286 1102 14 0.6180756645521228 1102 15 0.6991549180478884 1102 16 0.704647538243691 1102 17 0.6324146819791423 1102 18 0.5564975759204742 1102 19 0.6508402549758543 1102 20 0.6472535821517766 1102 21 0.5872267577804564 1102 22 0.5064010230723426 1102 23 0.5449608763174467 1102 24 0.5398162234262145 1102 25 0.5956693789831533 1103 1 0.3070432232673106 1103 2 0.33132167998505935 1103 3 0.2713908645351353 1103 4 0.12607793054073824 1103 5 0.1722520788934645 1103 6 0.2084464059786839 1103 7 0.3032971281254788 1103 8 0.2892984913587071 1103 9 0.44238979525851363 1103 10 0.3997861249736161 1103 11 0.5077111688172451 1103 12 0.4701463521178084 1103 13 0.40424066842997775 1103 14 0.5714084227039535 1103 15 0.5770071175164659 1103 16 0.6703643545485605 1103 17 0.7470126792554055 1103 18 0.7040349072020788 1103 19 0.7172180203520426 1103 20 0.6026247762613366 1103 21 0.4534014986484465 1103 22 0.6526518407947748 1103 23 0.700016339467787 1103 24 0.6748597388190518 1103 25 0.6682885917198575 1104 1 0.20145996894148865 1104 2 0.24573571683024653 1104 3 0.24727640884365343 1104 4 0.07116178924208327 1104 5 0.07765925933469475 1104 6 0.09523038708843501 1104 7 0.10688555989237614 1104 8 0.0882075488139904 1104 9 0.048723599655509986 1104 10 0.1396779762537932 1104 11 0.07809293482041824 1104 12 0.17463840834901134 1104 13 0.12348638733019612 1104 14 0.07326013731604775 1104 15 0.19605120754158634 1104 16 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2315 9 -0.9992307282598958 2315 10 -0.9644296988157208 2315 11 -1.0331935318532075 2315 12 -0.9607302390441674 2315 13 -0.900642064273633 2315 14 -1.074644290626125 2315 15 -1.039694411467579 2315 16 -1.0882205303304815 2315 17 -1.0561308599821189 2315 18 -1.1113964221912767 2315 19 -1.0628782636575451 2315 20 -0.9624036439014461 2315 21 -0.9457483440475016 2315 22 -0.9054414571678961 2315 23 -0.8550330169129552 2315 24 -1.043129216949417 2315 25 -1.0365615693497006 2316 1 -1.3860033783662082 2316 2 -1.3317514466439988 2316 3 -1.196649544865405 2316 4 -1.0880107606397615 2316 5 -1.120528199208728 2316 6 -1.1689642514102445 2316 7 -1.1640289698168615 2316 8 -1.1871878937805462 2316 9 -1.4709998445797439 2316 10 -1.3435544447731615 2316 11 -1.4853144214003322 2316 12 -1.4964826882662834 2316 13 -1.4646308421604672 2316 14 -1.9335028040636644 2316 15 -1.7760659554337863 2316 16 -1.8218280640111146 2316 17 -1.9759817082426494 2316 18 -2.1966271057682505 2316 19 -2.129153254435103 2316 20 -2.062240838863204 2316 21 -2.134816924989245 2316 22 -2.2211328136632886 2316 23 -2.306662836915379 2316 24 -2.2945391276380604 2316 25 -2.2695762803367288 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14502.0 3 0.0 4 275.0 5 2301.0 6 3188.0 7 2459.0 8 2017.0 9 1776.0 10 1663.0 11 1538.0 12 1515.0 13 1515.0 14 1499.0 15 1465.0 16 1536.0 17 1610.0 18 1866.0 19 2010.0 20 2626.0 21 3433.0 22 4457.0 23 5538.0 24 6731.0 25 8085.0 26 9035.0 27 10333.0 28 12323.0 29 15076.0 30 19187.0 31 25380.0 32 33205.0 33 43950.0 34 59932.0 35 82803.0 36 122984.0 37 235142.0 38 1155781.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78287703750593 18.688716569077386 13.219074748114155 24.309331645302525 2 15.840897898775713 20.096015337172894 40.012587858247024 24.05049890580437 3 19.149907701706663 24.62033933118629 29.527764537968697 26.701988429138346 4 12.918689518528778 16.834639299486707 37.22682941473265 33.01984176725187 5 13.566701185522328 37.53878098524267 34.96194137981216 13.932576449422845 6 32.40468505278929 36.520832554089196 17.54223854405763 13.53224384906388 7 28.618304779032748 31.582304920210735 21.392362587202427 18.407027713554086 8 26.060022787862696 35.66948433373947 20.04594446103755 18.224548417360285 9 26.787932620425657 14.80231721335917 19.88929173897422 38.52045842724095 10 15.925460380610806 28.297102747216858 33.26188012719338 22.51555674497896 11 34.76050915347781 22.38486174031192 23.75247019304779 19.102158913162473 12 23.891429938161362 24.612178281562308 30.682142657702222 20.814249122574104 13 29.55848383723717 21.49066687035286 25.612889121840336 23.337960170569627 14 21.562857498003805 22.064065749084065 27.33064996955671 29.042426783355417 15 23.689020215108133 29.8052755217057 23.668109850519862 22.83759441266631 16 22.64801040290177 27.78620751766602 26.688931011254535 22.876851068177665 17 22.07283526875695 27.965532623768713 27.170103274674613 22.791528832799727 18 21.909825946633745 26.46692943907711 29.515022688873817 22.108221925415332 19 23.610970666801492 26.322511397055727 28.101115689595606 21.96540224654718 20 24.044469583975868 25.81712254889569 28.45003202130259 21.688375845825856 21 23.61676399457323 25.81559521702859 27.76499734560255 22.802643442795627 22 23.7840570931978 26.763384510046613 27.731651436547022 21.72090696020857 23 22.914470018317544 26.532843601446444 28.20917815419392 22.343508226042093 24 23.387980214205662 25.8643118369273 28.470730001432532 22.276977947434503 25 22.92640999064641 26.43900995188378 28.434179369854473 22.20040068761534 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 295.0 1 295.0 2 551.5 3 808.0 4 808.0 5 808.0 6 2090.5 7 3373.0 8 3373.0 9 3373.0 10 4142.5 11 4912.0 12 4912.0 13 4912.0 14 6391.0 15 7870.0 16 7870.0 17 7870.0 18 14257.5 19 20645.0 20 20645.0 21 20645.0 22 32161.0 23 43677.0 24 43677.0 25 43677.0 26 63952.0 27 84227.0 28 84227.0 29 84227.0 30 106134.0 31 128041.0 32 128041.0 33 128041.0 34 145170.0 35 162299.0 36 162299.0 37 162299.0 38 175015.5 39 187732.0 40 187732.0 41 187732.0 42 203437.0 43 219142.0 44 219142.0 45 219142.0 46 236100.5 47 253059.0 48 253059.0 49 253059.0 50 248720.0 51 244381.0 52 244381.0 53 244381.0 54 221624.0 55 198867.0 56 198867.0 57 198867.0 58 172606.5 59 146346.0 60 146346.0 61 146346.0 62 122607.5 63 98869.0 64 98869.0 65 98869.0 66 76275.0 67 53681.0 68 53681.0 69 53681.0 70 39534.5 71 25388.0 72 25388.0 73 25388.0 74 17611.0 75 9834.0 76 9834.0 77 9834.0 78 6746.5 79 3659.0 80 3659.0 81 3659.0 82 2412.0 83 1165.0 84 1165.0 85 1165.0 86 751.0 87 337.0 88 337.0 89 337.0 90 224.5 91 112.0 92 112.0 93 112.0 94 62.0 95 12.0 96 12.0 97 12.0 98 8.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15989584649998737 2 0.004265995904643932 3 5.2666616106715206E-5 4 7.373326254940129E-4 5 0.11523455604149285 6 0.027860639920452342 7 0.022277978613140532 8 0.01880198195009733 9 0.03128396996738883 10 0.02759730683991877 11 0.0318633027445627 12 0.03001997118082767 13 0.020592646897725643 14 0.006530660397232685 15 0.008215992112647572 16 0.001843331563735032 17 6.846660093872977E-4 18 4.2133292885372165E-4 19 0.0 20 0.0 21 0.0 22 0.004529328985177507 23 5.26666161067152E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1898736.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.30252572285997 #Duplication Level Percentage of deduplicated Percentage of total 1 76.05581206411665 45.86357563369398 2 14.884144587625162 17.951030237160666 3 4.649053835624312 8.410490655290879 4 1.8944085241889546 4.569504750380386 5 0.8680571555514663 2.6173019475777477 6 0.4686548096846429 1.6956641229690133 7 0.2746498140313378 1.1593454232782419 8 0.17604505201000573 0.8492769021772474 9 0.12651045944048106 0.6866010211158398 >10 0.5302179059436731 5.443845109697986 >50 0.036082763448103114 1.507043871154669 >100 0.02973692162099892 3.569363185820804 >500 0.0035746102011470207 1.425469437554052 >1k 0.002789939669187915 2.804883063235666 >5k 1.743712293242447E-4 0.8225378729957261 >10k+ 8.718561466212235E-5 0.624066765897045 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 11870 0.6251527331867094 No Hit GGTATCAACGCAGAGTACTTTTTTT 7998 0.4212275956215082 No Hit TATCAACGCAGAGTACTTTTTTTTT 7647 0.4027416133680512 No Hit GTACTGGTTCACTATCGGTCAGTCA 3804 0.20034380766994464 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3661 0.19281248156668435 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2633 0.13867120020898113 No Hit GTCCTACAGTGGACATTTCTAAATT 2550 0.13429987107212377 No Hit CTGTAGGACGTGGAATATGGCAAGA 2520 0.13271987258892232 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2286 0.12039588441995096 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2284 0.12029055118773753 No Hit GGATACCACGTGTCCCGCCCTACTC 2260 0.11902655240117636 No Hit ATCATTAACTGAATCCATAGGTTAA 2075 0.10928322842143404 No Hit CTTTAGGACGTGAAATATGGCGAGG 2019 0.106333897919458 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1900 0.0 14.571946 1 CATTTCG 60 2.579621E-5 14.243679 14 TTTCGCA 55 1.9683434E-4 13.811689 16 TAACCTA 125 1.3969839E-9 12.167424 5 GTAAGAC 110 4.9307346E-7 11.234128 3 ACGAAAT 105 3.4744226E-6 10.8551855 12 CGAAATC 105 3.4788118E-6 10.854042 13 GTATCAA 4265 0.0 10.663187 1 GTGGTAT 515 0.0 10.345678 1 GTATAGG 130 3.7631435E-7 10.246201 1 TAGGACA 540 0.0 10.209933 4 CTGCGTC 75 0.0026502584 10.132307 9 GGCGAGG 640 0.0 10.089006 19 AACCATC 180 3.947207E-10 10.026763 7 TCCTATA 220 1.8189894E-12 9.938145 2 GTAGGAC 1340 0.0 9.931433 3 TGGTATC 550 0.0 9.678889 2 CCCCGTA 265 0.0 9.674697 15 CCCGTAC 265 0.0 9.674697 16 TAGGACG 1435 0.0 9.671407 4 >>END_MODULE