FastQCFastQC Report
Thu 26 May 2016
SRR1512371_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512371_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2390897
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65790.2751686919177196No Hit
GTCCTACAGTGGACATTTCTAAATT56000.23422171678662862No Hit
CTGTAGGACGTGGAATATGGCAAGA47670.1993812364146176No Hit
GTCCTAAAGTGTGTATTTCTCATTT44720.18704277097675057No Hit
GGTATCAACGCAGAGTACTTTTTTT41320.1728221667432767No Hit
CTTTAGGACGTGAAATATGGCGAGG39230.16408067767034715No Hit
TATCAACGCAGAGTACTTTTTTTTT36910.1543772065463297No Hit
GTCCTACAGTGTGCATTTCTCATTT32130.13438471000632818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11600.013.8283791
TAGGACC9100.013.3419484
GGCGAGG12150.011.70112519
TCGTAGA752.1047652E-411.3823492
ATAGGAC3450.010.9974393
GTCCTAC26700.010.9836491
AGGACCG951.3844938E-510.9829695
TGTAGGA30150.010.9796572
ACTCCGT700.00151104810.8405616
GTATCAA26750.010.7055961
AAATGTC7450.010.6952757
AGGACCT17700.010.6642545
GTATACG650.00819666810.5980591
TCCTACA29850.010.5180282
GGACGTG26700.010.4446986
GGACCTG17950.010.4102616
GCGTGCG1002.5205816E-510.4036099
GTCTAAG2650.010.3980951
GTAGGAC29300.010.3917363
GACGTGA14400.010.3420377
Produced by FastQC (version 0.11.5)