FastQCFastQC Report
Thu 26 May 2016
SRR1512371_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512371_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2390897
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77460.3239788246837902No Hit
GTCCTACAGTGGACATTTCTAAATT58300.24384153729750802No Hit
CTGTAGGACGTGGAATATGGCAAGA55560.2323814032975908No Hit
GGTATCAACGCAGAGTACTTTTTTT49660.20770447242185672No Hit
GTCCTAAAGTGTGTATTTCTCATTT49000.20494400218830003No Hit
TATCAACGCAGAGTACTTTTTTTTT48560.20310368869926226No Hit
CTTTAGGACGTGAAATATGGCGAGG45610.19076522326139522No Hit
GTCCTACAGTGTGCATTTCTCATTT35490.14843801301352588No Hit
CTGTAGGACCTGGAATATGGCGAGA25710.10753286318900396No Hit
CTGAAGGACCTGGAATATGGCGAGA25140.10514882071456864No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23940.10012978392628373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.013.6311961
TAGGACC9700.013.4279084
TTACACG550.003051548512.1002644
TGTAGGA31300.011.2688592
GGCGAGG13700.011.15838419
CTGTAGG30250.011.1047591
GTATACG600.00580352911.1021381
GGACGTG30150.011.058076
AGGACGT30550.011.01675
GACGTGA16000.010.9827567
ATCGCGC1752.0008883E-1110.8555418
GTAGGAC30950.010.8436013
GTCCTAA14550.010.7914741
ACCGTCT803.7774193E-410.6859248
GACGTGG14350.010.5907727
TTAGGAC18550.010.5580283
GTCCTAC28400.010.554851
TAGGACG31950.010.5042094
AGGACCT18300.010.4944525
GGACCTG18450.010.1936186
Produced by FastQC (version 0.11.5)