FastQCFastQC Report
Thu 26 May 2016
SRR1512370_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512370_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887640
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66650.35308639359199845No Hit
GTCCTACAGTGGACATTTCTAAATT54430.28834947341654127No Hit
GTCCTAAAGTGTGTATTTCTCATTT51420.2724036362865801No Hit
CTGTAGGACGTGGAATATGGCAAGA48900.2590536331080079No Hit
CTTTAGGACGTGAAATATGGCGAGG44500.23574410374859614No Hit
GGTATCAACGCAGAGTACTTTTTTT42600.2256786251615774No Hit
TATCAACGCAGAGTACTTTTTTTTT39280.20809052573583947No Hit
GTCCTACAGTGTGCATTTCTCATTT30360.1608357525799411No Hit
CTGAAGGACCTGGAATATGGCGAGA23510.12454705346358415No Hit
GTCCTTCAGTGTGCATTTCTCATTT20930.11087919306647454No Hit
CTGTAGGACCTGGAATATGGCGAGA19430.1029327626030387No Hit
ATTTAGAAATGTCCACTGTAGGACG19230.10187323854124726No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19100.10118454790108283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT350.002190581816.2631914
TAGACCG400.005326455514.231055
GGTATCA11950.014.1569231
TAGGACC10900.012.7071714
GGCGAGG14950.012.36131819
GACCTCG550.00309958712.074837
GTCGAGG802.9390721E-511.84626319
TTGGACG752.1010847E-411.38423254
AGGACCT20250.011.2911385
CACCTTT7500.011.26254414
ACCTTTT7800.011.19440415
TGGCGAG32800.011.12067218
GGACCTG19850.011.0407146
GTATAGA2250.010.9286331
TTAGGAC19450.010.8284793
TTTAGGA20650.010.7505312
TCGAACT1159.0977665E-710.71314219
CCACCTT8000.010.67243413
TAATACT2050.010.6437954
AAGGCGT1701.4188117E-1010.6035276
Produced by FastQC (version 0.11.5)