FastQCFastQC Report
Thu 26 May 2016
SRR1512370_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512370_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887640
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82880.4390667712063741No Hit
GGTATCAACGCAGAGTACTTTTTTT56890.30138161937657604No Hit
GTCCTACAGTGGACATTTCTAAATT56350.29852090440973916No Hit
CTGTAGGACGTGGAATATGGCAAGA56320.29836197580047047No Hit
TATCAACGCAGAGTACTTTTTTTTT54330.2878197113856456No Hit
GTCCTAAAGTGTGTATTTCTCATTT52110.27605899429976055No Hit
CTTTAGGACGTGAAATATGGCGAGG50590.2680066114301456No Hit
GTCCTACAGTGTGCATTTCTCATTT29910.15845182344091036No Hit
CTGAAGGACCTGGAATATGGCGAGA27900.14780360661990635No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27040.14324765315420313No Hit
CTGTAGGACCTGGAATATGGCGAGA23650.12528872030683816No Hit
ATTTAGAAATGTCCACTGTAGGACG21870.11585895615689433No Hit
TTTCTAAATTTTCCACCTTTTTCAG21000.11125002648810156No Hit
GTCCTTCAGTGTGCATTTCTCATTT20440.1082833591150855No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19980.10584645377296518No Hit
GAATATGGCAAGAAAACTGAAAATC19730.10452204869572587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG453.544535E-516.8861089
AATGTCG350.002157479316.3003145
CCGTCTA400.00528542714.2476549
GGTATCA13150.014.18331
TAGGACC9500.013.5117434
CACCTTT7150.012.08475614
CCACCTT7500.011.64863713
CACACCG752.0570718E-411.4102195
GGCGAGG12750.011.09572219
AGGACCG700.001480549610.8668765
AGGACCT19900.010.7508345
GTCCTAA15950.010.7388751
TGTAGGA31750.010.6310042
GTATCAA28700.010.4773671
GGACCTG19250.010.4606156
GTCGAGG1002.4140454E-510.44412819
TGGCGAA1106.086959E-610.35781318
GACGTGA16450.010.3356857
TAAGGGT1752.3283064E-1010.3232574
ACGAAAT1752.3646862E-1010.31094812
Produced by FastQC (version 0.11.5)