FastQCFastQC Report
Thu 26 May 2016
SRR1512369_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512369_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402610
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93230.3880363438094406No Hit
GTCCTACAGTGGACATTTCTAAATT79470.33076529274414074No Hit
CTGTAGGACGTGGAATATGGCAAGA74240.30899729877091997No Hit
GTCCTAAAGTGTGTATTTCTCATTT66420.276449361319565No Hit
GGTATCAACGCAGAGTACTTTTTTT57360.23874036984779054No Hit
CTTTAGGACGTGAAATATGGCGAGG56030.23320472319685676No Hit
TATCAACGCAGAGTACTTTTTTTTT55230.2298750109256184No Hit
GTCCTACAGTGTGCATTTCTCATTT39180.16307265848389876No Hit
GTACTGGTTCACTATCGGTCAGTCA31150.1296506715613437No Hit
CTGAAGGACCTGGAATATGGCGAGA28970.12057720562221917No Hit
CTGTAGGACCTGGAATATGGCGAGA28660.11928694211711431No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26470.11017185477459929No Hit
GTCCTTCAGTGTGCATTTCTCATTT24460.10180595269311292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC602.6910646E-514.1802448
GGTATCA15750.013.6776191
TAGGACC11950.013.3364594
TCGAACT1850.012.29477719
AGGACCG951.0587628E-611.98275855
GGACCGT658.1221515E-411.6757526
CCACCTT7950.011.45569413
CCCCGTA2600.011.31633415
GGCGAGG19000.011.27290719
GACGTGG20900.011.166227
TGTAGGA41650.010.9098722
GGACGTG42050.010.8965596
GGACCGC700.001509646810.8417716
ACCGTGC700.001554236610.8039978
TAGAAAT8900.010.7654094
AGGACGT43050.010.75345
CCCGTAC2650.010.74511316
CTACACT3900.010.7025494
TATACTG4000.010.6721445
CCAACGA1605.675247E-1010.66187619
Produced by FastQC (version 0.11.5)