FastQCFastQC Report
Thu 26 May 2016
SRR1512369_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512369_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402610
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT111920.4658267467462468No Hit
CTGTAGGACGTGGAATATGGCAAGA85950.3577359621411715No Hit
GTCCTACAGTGGACATTTCTAAATT84630.3522419368936282No Hit
GGTATCAACGCAGAGTACTTTTTTT74680.31082864052010106No Hit
TATCAACGCAGAGTACTTTTTTTTT74260.30908054157770093No Hit
GTCCTAAAGTGTGTATTTCTCATTT71190.2963027707368237No Hit
CTTTAGGACGTGAAATATGGCGAGG66210.2755753118483649No Hit
GTCCTACAGTGTGCATTTCTCATTT42950.17876392756210954No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35970.14971218799555486No Hit
CTGAAGGACCTGGAATATGGCGAGA35380.14725652519551655No Hit
GTACTGGTTCACTATCGGTCAGTCA33460.13926521574454448No Hit
CTGTAGGACCTGGAATATGGCGAGA33450.139223594341154No Hit
GTCCTTCAGTGTGCATTTCTCATTT25920.10788267758812292No Hit
GAATATGGCAAGAAAACTGAAAATC25400.10571836461181798No Hit
ATTTAGAAATGTCCACTGTAGGACG25090.10442810110671312No Hit
TTTCTAAATTTTCCACCTTTTTCAG24790.10317945900499874No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24760.1030545947948273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG350.002173207216.2828610
GGTATCA19650.013.8971881
TAGGACC13250.013.5607084
TCCAACG2200.012.51610318
TGTAGGA42000.011.4549182
GGCGAGG18050.011.25719919
CTGTAGG42100.011.2326411
AGGACCT26300.011.206025
CGCGCCT1950.011.20269812
ACCGCCC600.00588491511.081398
GGACCTG26150.011.0059476
GTAGGAC43400.010.9963983
CCAACGA1408.427378E-910.85139819
TTAGGAC23800.010.6652423
AGGACGT45550.010.6236775
GACGTGG23550.010.6074827
GGACGTG44300.010.5706016
CGTGCGC909.5360454E-510.55370510
TGGCGAG41200.010.53204618
ATCCCGT1451.4484613E-810.48092110
Produced by FastQC (version 0.11.5)