FastQCFastQC Report
Thu 26 May 2016
SRR1512368_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512368_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2540578
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT83550.3288621723088211No Hit
GTATCAACGCAGAGTACTTTTTTTT81020.3189038085034193No Hit
CTGTAGGACGTGGAATATGGCAAGA76490.30107322034592127No Hit
GTCCTAAAGTGTGTATTTCTCATTT71810.28265221536201607No Hit
CTTTAGGACGTGAAATATGGCGAGG58710.23108914585578555No Hit
GGTATCAACGCAGAGTACTTTTTTT50720.19963960956916102No Hit
TATCAACGCAGAGTACTTTTTTTTT45670.17976224308011798No Hit
GTCCTACAGTGTGCATTTCTCATTT42440.16704860075148253No Hit
CTGAAGGACCTGGAATATGGCGAGA32540.12808109020860606No Hit
GTCCTTCAGTGTGCATTTCTCATTT30050.11828017089024624No Hit
CTGTAGGACCTGGAATATGGCGAGA28250.11119516897335961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1650.013.78240418
TAGGACC13000.013.7191674
GGTATCA14000.013.2123671
AAATGTC9050.011.6357997
TGTAGGA45200.011.312632
GTCCTAA20800.011.3053861
GTCCTAT4200.011.2445681
GGCGAGG18800.011.19147419
GTAGGAC44050.011.1557063
AGGACGT44900.011.0079035
CCAACGT951.3973138E-510.97390619
GACGTGG22750.010.9672117
CTGTAGG41300.010.9348651
AATGTCC9700.010.9156368
AGGACCT26550.010.8265825
GGACGTG44650.010.7508376
ACCGAGC803.9465254E-410.6354368
CCAACGA1252.3163193E-710.61475919
GGACCTG25700.010.5940496
GTATCAA30750.010.59091951
Produced by FastQC (version 0.11.5)