FastQCFastQC Report
Thu 26 May 2016
SRR1512368_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512368_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2540578
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT99580.39195805049087257No Hit
CTGTAGGACGTGGAATATGGCAAGA90600.35661176314996035No Hit
GTCCTACAGTGGACATTTCTAAATT90060.35448626257489435No Hit
GTCCTAAAGTGTGTATTTCTCATTT77000.3030806375557058No Hit
CTTTAGGACGTGAAATATGGCGAGG68990.27155237902556034No Hit
GGTATCAACGCAGAGTACTTTTTTT65280.25694940285242174No Hit
TATCAACGCAGAGTACTTTTTTTTT64730.2547845411555953No Hit
GTCCTACAGTGTGCATTTCTCATTT45480.17901438176666884No Hit
CTGAAGGACCTGGAATATGGCGAGA41240.16232526614022477No Hit
CTGTAGGACCTGGAATATGGCGAGA33250.13087572985360024No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32320.12721514552987548No Hit
GTCCTTCAGTGTGCATTTCTCATTT30120.1185556987425696No Hit
ATTTAGAAATGTCCACTGTAGGACG28710.11300578057434174No Hit
GAATATGGCAAGAAAACTGAAAATC27670.1089122239112517No Hit
TTTCTAAATTTTCCACCTTTTTCAG27360.10769202913667678No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTC400.005293952314.24446413
GGATCGT551.9641139E-413.8155346
GCGTGCG903.652349E-813.7193219
GGTATCA16800.013.4830951
TAGGACC14200.013.4578544
TCCAACG3050.013.38639518
ATCCCGT1650.013.23962610
GCGCCAC1301.4551915E-1113.14873613
GACGTTG604.1009218E-412.6639897
CCAACGA1950.012.66000119
CCCGTTT1850.012.32050712
GGCGAGG19650.011.50030619
TCACGGT1251.7844286E-811.4209751
AATGTCC10100.011.3787828
AAATGTC10600.011.2004617
CACGGTA1201.262124E-711.0923462
CGTATAC600.005842801611.0921273
GGACGTC1201.2797682E-711.0812096
GGACCTG27950.010.9424466
AGGACCT29250.010.889195
Produced by FastQC (version 0.11.5)