FastQCFastQC Report
Thu 26 May 2016
SRR1512367_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512367_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1971805
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70950.3598225990906809No Hit
GGTATCAACGCAGAGTACTTTTTTT46030.23344093356087442No Hit
TATCAACGCAGAGTACTTTTTTTTT41630.2111263537723051No Hit
GTCCTACAGTGGACATTTCTAAATT34920.1770966195947368No Hit
CTGTAGGACGTGGAATATGGCAAGA33530.1700472409797115No Hit
GTCCTAAAGTGTGTATTTCTCATTT32370.16416430630817955No Hit
CTTTAGGACGTGAAATATGGCGAGG29080.14747908642081747No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20160.1022413473948996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12150.013.6040231
TAGGACC6750.011.946024
GGCGAGG9700.011.72516719
ATAAGAC1751.8189894E-1211.3838553
ACTATAC1201.3009594E-711.06763653
AATGTCC6850.010.902598
AGGACCT12550.010.884965
GTCTTAG2900.010.8564121
TGTCGAG1053.5807388E-610.82795718
TTTAGAA7550.010.8058662
ATTTAGA6650.010.6523441
AAATGTC6600.010.636437
GGACCTG12400.010.5576076
TGGCGAG21050.010.53218218
GGTCTAC1501.4186298E-810.4945321
TGTCCAC7500.010.36376210
TAGAAAT7150.010.3489584
TAAGACT1951.6370905E-1110.216284
GTCTTAC1353.9876977E-710.2030171
GAAATGT7600.010.1106616
Produced by FastQC (version 0.11.5)