FastQCFastQC Report
Thu 26 May 2016
SRR1512367_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512367_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1971805
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84290.42747634781329796No Hit
GGTATCAACGCAGAGTACTTTTTTT57690.29257457000058323No Hit
TATCAACGCAGAGTACTTTTTTTTT54530.27654864451606526No Hit
CTGTAGGACGTGGAATATGGCAAGA37970.19256468058454057No Hit
GTCCTACAGTGGACATTTCTAAATT36560.18541387206138538No Hit
GTCCTAAAGTGTGTATTTCTCATTT35020.1776037691353861No Hit
CTTTAGGACGTGAAATATGGCGAGG33090.16781578300085453No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27440.1391618339541689No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20560.10426994555749682No Hit
GTCCTACAGTGTGCATTTCTCATTT20280.10284992684367876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.014.4322521
TAGAACG655.411987E-513.1646764
TAGGACC6400.012.0333374
GGCGAGG9500.011.69330119
GTACTAA1552.5465852E-1111.6636331
GTCCCGC2750.011.05157912
TGGCGAG19450.010.9346118
TGCGATG700.001495052810.8542312
TGTAGGA22450.010.7992552
GTAGAAC4350.010.709963
CGCCCTA2400.010.68138116
GTAGGAC21850.010.6609433
GGACCTG11850.010.6609376
CTGTAGG22200.010.6294471
TGTAGAA5200.010.6046082
AGGACCT12850.010.5069645
AGGACGT22450.010.4186345
TGGTATC3950.010.3500232
TCCCGCC2600.010.22698613
GACGTGG11300.010.1711267
Produced by FastQC (version 0.11.5)