FastQCFastQC Report
Thu 26 May 2016
SRR1512366_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512366_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072992
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT77050.3716849847949244No Hit
CTGTAGGACGTGGAATATGGCAAGA71930.34698638489680617No Hit
GTCCTAAAGTGTGTATTTCTCATTT71520.34500856732683965No Hit
GTATCAACGCAGAGTACTTTTTTTT69100.3333346197187447No Hit
CTTTAGGACGTGAAATATGGCGAGG59410.28659058983343877No Hit
GGTATCAACGCAGAGTACTTTTTTT44150.21297718466834412No Hit
TATCAACGCAGAGTACTTTTTTTTT39990.192909572251123No Hit
GTCCTACAGTGTGCATTTCTCATTT39490.1904975996048224No Hit
CTGAAGGACCTGGAATATGGCGAGA33530.16174688566091908No Hit
GTCCTTCAGTGTGCATTTCTCATTT29820.14385004862536854No Hit
CTGTAGGACCTGGAATATGGCGAGA26500.12783455025393248No Hit
ATTTAGAAATGTCCACTGTAGGACG26370.12720743736589432No Hit
TTTCTAAATTTTCCACCTTTTTCAG24080.11616060264583751No Hit
GAATATGGCAAGAAAACTGAAAATC24010.11582292647535544No Hit
GGAATATGGCGAGAAAACTGAAAAT21060.10159228786218182No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20820.1004345409919575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATCG350.002192549616.2611127
GTCGTAC506.468642E-515.7402981
ACCGTCC456.997905E-414.7115558
GGTATCA13550.014.3027611
TAGGACC12300.013.1867284
CGGTTTC1103.8906364E-812.0741113
TGTCCAC11300.011.32856510
ACCTTTT9400.011.30671415
AAATGTC10650.011.2224597
AGGCGAT600.005925032311.07115714
GGTCTAT1353.40533E-810.9307631
GGACCTG24600.010.91235356
GAAATGT11050.010.9020596
ATGTCCA11200.010.8968849
AATGTCC11200.010.892938
AATCCCG2350.010.88625719
AGGACCT25800.010.8824985
TAGCACC1408.560164E-910.8402174
CCACCTT9650.010.8139713
ACTGTTC5250.010.808498
Produced by FastQC (version 0.11.5)