FastQCFastQC Report
Thu 26 May 2016
SRR1512366_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512366_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072992
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83810.40429485497290873No Hit
CTGTAGGACGTGGAATATGGCAAGA82670.3987955573393433No Hit
GTCCTACAGTGGACATTTCTAAATT81150.39146316049458946No Hit
GTCCTAAAGTGTGTATTTCTCATTT77160.37221561877711057No Hit
CTTTAGGACGTGAAATATGGCGAGG72470.3495913153548108No Hit
GGTATCAACGCAGAGTACTTTTTTT56440.27226347231441317No Hit
TATCAACGCAGAGTACTTTTTTTTT54340.2621331871999506No Hit
GTCCTACAGTGTGCATTTCTCATTT42370.20439056204751396No Hit
CTGAAGGACCTGGAATATGGCGAGA40280.19430851638597735No Hit
ATTTAGAAATGTCCACTGTAGGACG31360.15127892437597446No Hit
TTTCTAAATTTTCCACCTTTTTCAG30170.14553842947777898No Hit
GTCCTTCAGTGTGCATTTCTCATTT30080.14510427440144485No Hit
CTGTAGGACCTGGAATATGGCGAGA29990.14467011932511076No Hit
GAATATGGCAAGAAAACTGAAAATC27510.13270673499945973No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26730.12894405767123077No Hit
GGAATATGGCGAGAAAACTGAAAAT24290.11717363115728377No Hit
ATTCCAGGTCCTTCAGTGTGCATTT22500.10853876908352758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC551.1308051E-515.54235610
GCGTGCG707.278504E-613.569059
GGTATCA15500.013.3812281
TAGGACC12200.013.3261034
TCCAACG1403.6379788E-1212.88593418
AATCCCG2650.012.18213419
GCGCCAC550.003076403612.0870413
GGCGTGC951.042521E-611.9978968
CGATAAG951.0431304E-611.99731712
AGGACCT25000.011.9414365
GGACCTG25300.011.6761026
GTGCGCC752.0782958E-411.39772811
CCACCTT9650.011.31762113
CACCTTT9700.011.25765314
AAGGCGT1104.9803566E-711.2255766
ATAAGCG1105.000584E-711.22205514
GGCGAGG18850.011.13194919
ACCGTGC600.005884450411.081398
ATGTCGA1900.010.99409217
CCAACGA951.3688159E-510.99409219
Produced by FastQC (version 0.11.5)