FastQCFastQC Report
Thu 26 May 2016
SRR1512365_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512365_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2130662
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70090.3289587930887208No Hit
GTCCTACAGTGGACATTTCTAAATT68730.32257580038504463No Hit
CTGTAGGACGTGGAATATGGCAAGA64640.3033798885041363No Hit
GTCCTAAAGTGTGTATTTCTCATTT63440.29774783611853967No Hit
CTTTAGGACGTGAAATATGGCGAGG51980.24396173583609224No Hit
GGTATCAACGCAGAGTACTTTTTTT44020.20660245501163488No Hit
TATCAACGCAGAGTACTTTTTTTTT41290.19378953583440264No Hit
GTCCTACAGTGTGCATTTCTCATTT37350.17529763050169384No Hit
CTGAAGGACCTGGAATATGGCGAGA29360.13779754836759653No Hit
CTGTAGGACCTGGAATATGGCGAGA25660.12043205351200707No Hit
GTCCTTCAGTGTGCATTTCTCATTT25530.11982191450356744No Hit
ATTTAGAAATGTCCACTGTAGGACG24200.11357972310953121No Hit
GAATATGGCAAGAAAACTGAAAATC22590.10602338615885579No Hit
TTTCTAAATTTTCCACCTTTTTCAG21650.10161161179013847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2100.014.4391518
GGTATCA12350.013.3835111
CCAACGT1301.4551915E-1113.12390219
TAGGACC10100.013.055584
TACGGCG658.0998655E-411.67946316
TGGACCG658.1207446E-411.67587955
GTCCCGC1604.7293724E-1111.258224512
TCTATAC2800.011.1804333
AATCCCG2250.010.95278819
ACGGCGA700.001504935810.84572817
CGTGCTA700.001520418610.83242819
GTCTTAT2001.8189894E-1210.8223221
CCACCTT9100.010.73738613
ATACGGC1158.9433524E-710.72758715
CCAACGA1159.0830326E-710.714684519
GGCGAGG17250.010.65973719
TGTAGGA37550.010.5853892
ATTAGGC909.656834E-510.5404763
GGCGAAA909.740337E-510.53152719
AGGACCT22100.010.4739515
Produced by FastQC (version 0.11.5)