FastQCFastQC Report
Thu 26 May 2016
SRR1512365_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512365_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2130662
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86130.4042405599761952No Hit
CTGTAGGACGTGGAATATGGCAAGA74070.3476384335009495No Hit
GTCCTACAGTGGACATTTCTAAATT73540.345150943697311No Hit
GTCCTAAAGTGTGTATTTCTCATTT68120.31971284042236636No Hit
CTTTAGGACGTGAAATATGGCGAGG62800.2947440748462215No Hit
GGTATCAACGCAGAGTACTTTTTTT57830.2714179912158756No Hit
TATCAACGCAGAGTACTTTTTTTTT54530.255929847155485No Hit
GTCCTACAGTGTGCATTTCTCATTT41070.1927569928970433No Hit
CTGAAGGACCTGGAATATGGCGAGA35440.16633328045461926No Hit
CTGTAGGACCTGGAATATGGCGAGA30060.14108291225919456No Hit
ATTTAGAAATGTCCACTGTAGGACG28460.1335735090783991No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27570.12939640355908164No Hit
GTCCTTCAGTGTGCATTTCTCATTT26780.1256886357385639No Hit
TTTCTAAATTTTCCACCTTTTTCAG26750.12554783442892398No Hit
GAATATGGCAAGAAAACTGAAAATC25220.11836696763728831No Hit
GGAATATGGCGAGAAAACTGAAAAT21600.10137694294073861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11000.013.9139164
GGTATCA15650.013.5595641
GTCTTAG2900.012.4692631
AGAGCGC550.00307249712.0891178
GCGCCAC658.0498087E-411.68810513
AAATGTC9800.011.4370787
TCGGTAA752.0422963E-411.419221
CTAGGAC3400.011.1840033
AGGACCT24500.011.1751365
AGCGTCG600.005896736411.07830917
GGACCTG24300.011.0230576
GTGGTAT4600.010.9641071
AATGTCC10750.010.9565148
TGTCCAC10950.010.92911710
TGGTATC4450.010.8952342
TTTAGAA11650.010.8530582
ACCTTTT8600.010.82067415
TAGAAAT11700.010.6439214
AGGACGT40550.010.643675
GAAATGT10900.010.6314516
Produced by FastQC (version 0.11.5)