FastQCFastQC Report
Thu 26 May 2016
SRR1512364_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512364_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037840
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69340.34026223844855336No Hit
GTCCTACAGTGGACATTTCTAAATT65480.3213206139834334No Hit
GTCCTAAAGTGTGTATTTCTCATTT58300.2860872296156715No Hit
CTGTAGGACGTGGAATATGGCAAGA56440.27695991834491424No Hit
CTTTAGGACGTGAAATATGGCGAGG49730.24403289757782748No Hit
GGTATCAACGCAGAGTACTTTTTTT43400.21297059631766968No Hit
TATCAACGCAGAGTACTTTTTTTTT39200.1923605386095081No Hit
GTCCTACAGTGTGCATTTCTCATTT35580.1745966317277117No Hit
CTGTAGGACCTGGAATATGGCGAGA27030.13264044282181134No Hit
CTGAAGGACCTGGAATATGGCGAGA25100.12316963058925136No Hit
GTCCTTCAGTGTGCATTTCTCATTT22050.1082028029678483No Hit
ATTTAGAAATGTCCACTGTAGGACG21710.10653436972480666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.014.556661
TAAACCG400.00533418314.2280195
TAGGACC12250.013.5504954
TTTCGCA500.001513143113.284416
GGCGAGG15750.012.51340519
TAGGACT3800.011.4822614
GGACGTC855.4088523E-511.1592316
AGGACCT23400.011.1473075
GGACCTG23050.011.0285156
ATAGGAC3700.011.023513
TGTAGGA38850.010.9868882
GTCCTAG3500.010.9620561
AGGACGT37350.010.9456065
CCAACGA1303.3951437E-810.93303219
GGACGTG36500.010.8626716
GTGGTAT4000.010.8215171
TTAGGAC21800.010.7471593
GACGTGA19250.010.6432977
CACCTTT7950.010.62405814
GTAGGAC38950.010.6177433
Produced by FastQC (version 0.11.5)