FastQCFastQC Report
Thu 26 May 2016
SRR1512364_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512364_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037840
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85320.418678600871511No Hit
GTCCTACAGTGGACATTTCTAAATT66520.326424056844502No Hit
CTGTAGGACGTGGAATATGGCAAGA64380.31592274172653395No Hit
GTCCTAAAGTGTGTATTTCTCATTT63940.3137635928237742No Hit
CTTTAGGACGTGAAATATGGCGAGG57510.28221057590389825No Hit
GGTATCAACGCAGAGTACTTTTTTT56200.2757822007615907No Hit
TATCAACGCAGAGTACTTTTTTTTT53220.26115887410199035No Hit
GTCCTACAGTGTGCATTTCTCATTT39620.19442154438032427No Hit
CTGTAGGACCTGGAATATGGCGAGA31280.15349585835983198No Hit
CTGAAGGACCTGGAATATGGCGAGA30970.15197463981470577No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26600.13053036548502336No Hit
TTTCTAAATTTTCCACCTTTTTCAG23930.1174282573705492No Hit
ATTTAGAAATGTCCACTGTAGGACG23450.11507282220390216No Hit
GTCCTTCAGTGTGCATTTCTCATTT22690.11134338319004436No Hit
GAATATGGCAAGAAAACTGAAAATC22510.11046009500255173No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG307.7476486E-418.99131216
ACGGCGA400.00529589314.243484517
GGTATCA15450.013.9813981
TAGGACC11300.013.209294
AGCCGGC751.4823421E-512.6639837
CCGCCCA604.1003188E-412.6639829
TCCAACG1352.7284841E-1112.66087418
GGACCGG550.003073883212.08834656
AGGACTG2850.011.6756585
ATAGGAC3200.011.5870493
GGCGAGG15150.011.28196719
CCAACGA1354.7639332E-911.2541119
AGGACCT21450.011.1694415
CTAGGAC3600.011.0918753
CCGCCCT2500.011.0144215
GGACCTG20550.011.0001026
ACTCCGT700.001494385810.8548426
ACCTTTT8700.010.80487215
CTCGAAC1252.2550194E-710.63513518
CGCCCTA2500.010.63513516
Produced by FastQC (version 0.11.5)