FastQCFastQC Report
Thu 26 May 2016
SRR1512363_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512363_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237744
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72970.32608734511186266No Hit
GGTATCAACGCAGAGTACTTTTTTT46940.20976483458340187No Hit
TATCAACGCAGAGTACTTTTTTTTT44810.200246319507504No Hit
GTCCTAAAGTGTGTATTTCTCATTT38820.1734782888480541No Hit
GTCCTACAGTGGACATTTCTAAATT38190.17066295340307022No Hit
CTTTAGGACGTGAAATATGGCGAGG35880.16034005677146268No Hit
CTGTAGGACGTGGAATATGGCAAGA35120.1569437790917996No Hit
GTCCTACAGTGTGCATTTCTCATTT26210.11712689208417049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14500.013.9831711
TAGGACC7950.012.7674514
TGGACCG802.9109608E-511.8576215
GGCGTGC1451.05501385E-1011.7349588
ATACGGC658.095945E-411.68018415
GGACCTG15600.011.0673626
AAGGCGT1900.010.9841486
AGGACCT16250.010.9746855
GGCGAGG12250.010.75462919
GTGTAGG4150.010.6726151
TGGTATC4350.010.4674172
TGTAGGA26250.010.2991912
GACGTGA15250.010.2016717
TGGCGAG27200.010.1368818
GTGGTAT4500.010.0612451
GCGCCAC1054.174006E-59.937592513
GTCCTAA12800.09.9194161
GTATCAA29200.09.7751081
GTAGGAC25550.09.7645543
AGGACGT27650.09.7090985
Produced by FastQC (version 0.11.5)