FastQCFastQC Report
Thu 26 May 2016
SRR1512363_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512363_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237744
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86750.3876672219878592No Hit
GGTATCAACGCAGAGTACTTTTTTT62350.2786288333249916No Hit
TATCAACGCAGAGTACTTTTTTTTT61410.27442817408961884No Hit
CTTTAGGACGTGAAATATGGCGAGG43920.19626909959316166No Hit
GTCCTAAAGTGTGTATTTCTCATTT42180.18849341122130145No Hit
CTGTAGGACGTGGAATATGGCAAGA39310.1756679941941527No Hit
GTCCTACAGTGGACATTTCTAAATT38210.17075232913148242No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27850.1244557018139698No Hit
GTCCTACAGTGTGCATTTCTCATTT26220.11717157994837657No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23070.1030949027234572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG400.005249755514.2623695
GGTATCA16000.013.9157751
CAGCGCA1001.4457146E-712.348569
TAGAACC1657.8216544E-1110.9486454
TAGGACC8850.010.8509764
TAAGACT1855.456968E-1210.7929044
GGCGAGG12000.010.6812419
TGTAGGA24800.010.4281652
AGGGACG750.002629355310.1421295
GTATCAA34150.010.1419681
GCCGGTT750.002651401810.13192711
CTGTAGG25250.010.0991531
AGGACGT27700.010.09178455
TGGTATC5000.010.0785162
GACGTGA14800.010.07652957
TGGCGAG24600.010.07336518
GGACGTG27350.010.0374476
GTCTTAC2201.8189894E-129.9475631
TTAGGAC17750.09.9095593
CGTGATT2500.09.87421418
Produced by FastQC (version 0.11.5)