FastQCFastQC Report
Thu 26 May 2016
SRR1512362_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512362_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272335
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74170.3264043373886333No Hit
GTCCTACAGTGGACATTTCTAAATT66240.29150631398979465No Hit
CTGTAGGACGTGGAATATGGCAAGA59130.2602169134392596No Hit
GTCCTAAAGTGTGTATTTCTCATTT49370.21726550002530437No Hit
GGTATCAACGCAGAGTACTTTTTTT44820.19724204397679038No Hit
GTCCTACAGTGTGCATTTCTCATTT44450.19561376293548266No Hit
CTTTAGGACGTGAAATATGGCGAGG41760.18377571968921835No Hit
TATCAACGCAGAGTACTTTTTTTTT40980.18034312722375884No Hit
CTGTAGGACCTGGAATATGGCGAGA30550.13444320489716524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13700.013.8587981
ATTTCGC500.00151078913.28725415
AAACGCC500.001528020213.2672719
GCGCCAC957.516064E-812.98351713
TATTGCG604.1311656E-412.65284616
TAGGACC12250.012.3894114
ACCGCTT550.003183218912.0321478
CATTTCG658.090696E-411.68110314
TTGCGAG907.70055E-611.57869718
CCAACGA1501.8371793E-1011.37194519
GTTTAGG2550.011.187851
TGGTATC4600.011.1353212
TTACACG600.0059435811.0665844
GGCGTGC1201.3649878E-711.02946958
TGTAGGA37650.011.0098962
GGCGAGG13150.010.95396519
TAGGACA11000.010.8653744
TCCAACG2100.010.82683218
GTCCTAC32350.010.8259121
CCTACAC3950.010.8064293
Produced by FastQC (version 0.11.5)