FastQCFastQC Report
Thu 26 May 2016
SRR1512362_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512362_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272335
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92620.4075983514754647No Hit
CTGTAGGACGTGGAATATGGCAAGA70600.31069362571979925No Hit
GTCCTACAGTGGACATTTCTAAATT69920.3077011092114499No Hit
GGTATCAACGCAGAGTACTTTTTTT60430.26593790088169217No Hit
TATCAACGCAGAGTACTTTTTTTTT56790.24991913604288102No Hit
GTCCTAAAGTGTGTATTTCTCATTT50940.22417469255193445No Hit
CTTTAGGACGTGAAATATGGCGAGG47020.20692371503321472No Hit
GTCCTACAGTGTGCATTTCTCATTT46330.2038871909291544No Hit
CTGTAGGACCTGGAATATGGCGAGA36940.16256405855650685No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30180.13281492385585753No Hit
CTGAAGGACCTGGAATATGGCGAGA25890.11393566529583005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.014.0592191
TAGGACC14050.013.2631514
TGGACCG604.066107E-412.6766855
GTCGAGG1052.7413807E-711.75540719
TGTAGGA38800.011.7381452
GGCGAGG12300.011.50177319
AGGACCT22950.011.4753015
CTGTAGG38700.011.335231
GTCCTAG3900.011.2236361
GTAGGAC39550.011.178243
GGACCTG22150.011.106376
GATCTAC1201.2473356E-711.1016411
GACGTGG18650.011.0516477
GCCGGTT1553.1468517E-1011.03004611
GGACGTG34350.010.9223596
CTAGGAC4450.010.8962523
AATCCCG2100.010.85114519
AGGACGT35650.010.6942865
CCACCTT7900.010.57875913
CGATAAG1002.4056082E-510.44767612
Produced by FastQC (version 0.11.5)