FastQCFastQC Report
Thu 26 May 2016
SRR1512361_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512361_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235607
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT77380.3461252357860751No Hit
CTGTAGGACGTGGAATATGGCAAGA71480.3197341929954594No Hit
GTATCAACGCAGAGTACTTTTTTTT65480.2928958443948333No Hit
GTCCTAAAGTGTGTATTTCTCATTT62330.27880571137950455No Hit
CTTTAGGACGTGAAATATGGCGAGG51680.23116764261339315No Hit
GTCCTACAGTGTGCATTTCTCATTT44270.19802228209161984No Hit
GGTATCAACGCAGAGTACTTTTTTT40090.17932489923318365No Hit
TATCAACGCAGAGTACTTTTTTTTT36020.16111955276575893No Hit
CTGAAGGACCTGGAATATGGCGAGA28800.12882407328300546No Hit
CTGTAGGACCTGGAATATGGCGAGA28670.1282425757299919No Hit
GTCCTTCAGTGTGCATTTCTCATTT27370.12242760019985624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC1000.017.06709910
GCGTGCG1203.6379788E-1214.1900429
GTGCGCC1351.8189894E-1213.34524411
TAGGACC11650.013.271944
GGTATCA11300.013.231631
GGCGTGC1600.013.0008328
CGCCACC1650.012.07796514
GCGCCAC1603.6379788E-1211.85775113
TGCGCCA1603.6379788E-1211.85321812
GGCGAGG16500.011.7170619
GTTCTAG2200.011.6251581
TAGAAAT8600.011.5814694
TGTAGGA42350.011.3339072
GACGTGG20200.011.1770617
GACGGGG855.4032007E-511.1604877
GGACGTG38400.011.1163916
GTAGGAC41600.011.0821873
AGGACGT39550.011.08097655
AATACCG600.00594192811.0669855
AAGGCGT2150.011.0302176
Produced by FastQC (version 0.11.5)