FastQCFastQC Report
Thu 26 May 2016
SRR1512361_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512361_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235607
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA83050.3714874752136668No Hit
GTCCTACAGTGGACATTTCTAAATT80050.35806830091335373No Hit
GTATCAACGCAGAGTACTTTTTTTT78310.3502851798191721No Hit
GTCCTAAAGTGTGTATTTCTCATTT65750.29410357008186144No Hit
CTTTAGGACGTGAAATATGGCGAGG60860.27223031597235114No Hit
GGTATCAACGCAGAGTACTTTTTTT53160.23778776860154757No Hit
TATCAACGCAGAGTACTTTTTTTTT50050.22387655791022304No Hit
GTCCTACAGTGTGCATTTCTCATTT46400.20754989584484215No Hit
CTGAAGGACCTGGAATATGGCGAGA35620.15933032952571716No Hit
CTGTAGGACCTGGAATATGGCGAGA35090.15695960873266188No Hit
GTCCTTCAGTGTGCATTTCTCATTT26980.12068310754081554No Hit
ATTTAGAAATGTCCACTGTAGGACG25530.11419717329566423No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24840.11111076320659222No Hit
TTTCTAAATTTTCCACCTTTTTCAG24570.10990303751956404No Hit
GAATATGGCAAGAAAACTGAAAATC23690.1059667463914722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13050.014.4983054
GGTATCA14200.013.8067491
CCCCGTA852.7184433E-713.40459615
TCCAACG1650.013.234755518
CCAACGA1301.4551915E-1113.14593419
CGTGCGC957.4094714E-812.99770410
CCCGTAC905.4466364E-712.6593316
TGTAGGA42100.011.6305372
CTGTAGG41700.011.5713621
AGGACCT25500.011.5215895
GTAGGAC42150.011.5039573
GGACCTG24450.011.498996
CCGTACA1001.9441359E-611.39339717
GTCGAGG1251.8493665E-811.39314219
CACCTTT8450.011.34921514
ACCTTTT8550.011.32727715
CCACCTT8100.011.25475113
GGACCGT855.334639E-511.1744966
TCGAGCA600.005884755411.0813769
AGGACGT39200.011.0361975
Produced by FastQC (version 0.11.5)