FastQCFastQC Report
Thu 26 May 2016
SRR1512360_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512360_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817004
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85310.4695091480260913No Hit
GGTATCAACGCAGAGTACTTTTTTT52980.29157888480157446No Hit
TATCAACGCAGAGTACTTTTTTTTT49920.27473797526037363No Hit
GTCCTACAGTGGACATTTCTAAATT44270.24364283182645718No Hit
CTGTAGGACGTGGAATATGGCAAGA41670.22933356228164603No Hit
GTCCTAAAGTGTGTATTTCTCATTT40400.22234403446552675No Hit
CTTTAGGACGTGAAATATGGCGAGG34870.1919093188567554No Hit
GTACTGGTTCACTATCGGTCAGTCA24430.13445209806912917No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24310.13379167024398403No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21020.11568494070458843No Hit
GTCCTACAGTGTGCATTTCTCATTT19230.10583355897950691No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCA457.0032425E-414.7099119
GGTATCA15400.014.3136331
TAGGACC5900.013.1842294
GTCCTAG2650.012.6257051
TCCAACG1157.3196134E-811.53693818
ATGTCGA1001.9509243E-611.38976317
TAATACC951.38249325E-510.9840374
TGTAGGA24300.010.9693512
CTACGGT700.001509782610.8413884
CCGGTTT1053.5579942E-610.8335912
ACCGTCC700.00155391310.8040038
CTGTAGG21650.010.7269611
TGTCGAG1159.100022E-710.71287118
GTAGGAC24050.010.6892483
GTCCTAC21750.010.6323981
GTCTTAC1957.2759576E-1210.5975931
GGACCTG13150.010.4606636
AATCCCG1553.792593E-910.39732619
AGGACCT14300.010.349175
GATCAAC2001.2732926E-1110.3326531
Produced by FastQC (version 0.11.5)