FastQCFastQC Report
Thu 26 May 2016
SRR1512360_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512360_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817004
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT105900.5828275556905763No Hit
GGTATCAACGCAGAGTACTTTTTTT70850.38992759509610325No Hit
TATCAACGCAGAGTACTTTTTTTTT69150.3805715342398806No Hit
CTGTAGGACGTGGAATATGGCAAGA49900.2746279039561828No Hit
GTCCTACAGTGGACATTTCTAAATT47570.2618045970179482No Hit
GTCCTAAAGTGTGTATTTCTCATTT44150.24298240400131205No Hit
CTTTAGGACGTGAAATATGGCGAGG41460.22817781358764208No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32730.18013168930833393No Hit
GTACTGGTTCACTATCGGTCAGTCA26820.14760561891993634No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25230.1388549502367634No Hit
GTCCTACAGTGTGCATTTCTCATTT21770.11981261461174549No Hit
CTGAAGGACCTGGAATATGGCGAGA21670.11926225809079122No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20160.11095187462438168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16700.014.24558451
TAGGACC6250.013.6898994
ATTGCGA550.003080028812.08500817
GGCGAGG11000.011.48075819
GTTATAC1354.6748028E-911.2674063
AATGCCG1354.7657522E-911.25376318
ATTTCCG1105.000802E-711.22179314
GTAGGAC21750.011.145993
GGACGTA600.005883218311.0815836
TATTGCG600.00589769911.07792416
CGCCCTA3350.011.05430316
TGTAGGA22450.011.0522322
TGTCGAG1408.416464E-910.85184418
AGGACGT26800.010.7842155
AGGACCG1158.732877E-710.7471985
TCCCGCC3550.010.700767513
CCCGTTT803.7806877E-410.68463512
GACGTGG12450.010.6810447
CTGTAGG22650.010.5873941
GCGATTT1355.705442E-810.55330811
Produced by FastQC (version 0.11.5)