##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512360_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1817004 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87746257025301 34.0 31.0 34.0 30.0 34.0 2 32.03559595906228 34.0 31.0 34.0 30.0 34.0 3 32.15776024708806 34.0 31.0 34.0 30.0 34.0 4 35.603192398035446 37.0 35.0 37.0 33.0 37.0 5 35.40389729466749 37.0 35.0 37.0 33.0 37.0 6 35.43575633295249 37.0 35.0 37.0 33.0 37.0 7 35.34724414475697 37.0 35.0 37.0 33.0 37.0 8 35.31367404804833 37.0 35.0 37.0 33.0 37.0 9 36.94949158064594 39.0 37.0 39.0 33.0 39.0 10 36.88255556949792 39.0 37.0 39.0 33.0 39.0 11 36.920215365513776 39.0 37.0 39.0 33.0 39.0 12 36.848465936233495 39.0 37.0 39.0 33.0 39.0 13 36.82649240177787 39.0 37.0 39.0 33.0 39.0 14 38.13744438647356 40.0 38.0 41.0 33.0 41.0 15 38.127170881296905 40.0 38.0 41.0 33.0 41.0 16 38.081369110910046 40.0 38.0 41.0 33.0 41.0 17 38.08333553475942 40.0 38.0 41.0 33.0 41.0 18 38.03909017261382 40.0 38.0 41.0 33.0 41.0 19 38.06384796070895 40.0 38.0 41.0 33.0 41.0 20 38.047046676837255 40.0 38.0 41.0 33.0 41.0 21 37.99930985292272 40.0 38.0 41.0 33.0 41.0 22 37.93959341861658 40.0 38.0 41.0 33.0 41.0 23 37.86445104138461 40.0 38.0 41.0 33.0 41.0 24 37.80693438209272 40.0 38.0 41.0 33.0 41.0 25 37.74165934692494 40.0 38.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34377386365305185 1101 2 0.32688790045791194 1101 3 0.3585367892620468 1101 4 0.27007504283366757 1101 5 0.1833939461164391 1101 6 0.4036962723892117 1101 7 0.4620662337708481 1101 8 0.46094289166336466 1101 9 0.7038383059926829 1101 10 0.6721388596471272 1101 11 0.659264312352434 1101 12 0.651974985442024 1101 13 0.6464452305292028 1101 14 0.8363088289161809 1101 15 0.8517703625531965 1101 16 0.8460428983432138 1101 17 0.7678622287252068 1101 18 0.6683660965933527 1101 19 0.7025854276486285 1101 20 0.8572008282530348 1101 21 0.894197799751737 1101 22 0.8129388104287685 1101 23 0.7357624284140414 1101 24 0.8677174475530265 1101 25 0.8365717933693659 1102 1 0.42286173719666564 1102 2 0.5176925271985198 1102 3 0.49698530687760467 1102 4 0.3822739236239343 1102 5 0.341462319809537 1102 6 0.41230930288747203 1102 7 0.45941347932748755 1102 8 0.484346080863709 1102 9 0.5148527108703789 1102 10 0.46352709236527545 1102 11 0.435243410331708 1102 12 0.5064545613381739 1102 13 0.5791157963238334 1102 14 0.7225330329845789 1102 15 0.6849415831149912 1102 16 0.8083211512307216 1102 17 0.7608408508929685 1102 18 0.698708554559822 1102 19 0.7924001138025147 1102 20 0.66813101870396 1102 21 0.680229994927096 1102 22 0.7165153971338398 1102 23 0.75029573311447 1102 24 0.7467581610176524 1102 25 0.802212307245803 1103 1 0.4887982844302634 1103 2 0.45219778420820234 1103 3 0.4645918971914327 1103 4 0.33096962187912027 1103 5 0.3857922202797397 1103 6 0.4126119992757893 1103 7 0.5350749040568914 1103 8 0.6337473410714338 1103 9 0.740441874397284 1103 10 0.6899491749740321 1103 11 0.7248786367030959 1103 12 0.6803457162098567 1103 13 0.7523189001282446 1103 14 0.942551834145597 1103 15 0.9435664141263089 1103 16 0.9112503076035026 1103 17 0.9103541462054139 1103 18 0.9333083357889649 1103 19 0.9243382980734793 1103 20 0.9345193928585829 1103 21 0.8504441519018116 1103 22 0.9648666954450178 1103 23 0.9879282383864592 1103 24 0.9854286470541638 1103 25 1.0098718219062732 1104 1 0.34055173442898123 1104 2 0.3043167354763412 1104 3 0.346382558388143 1104 4 0.22157793290813999 1104 5 0.15307854545130084 1104 6 0.19755274600198902 1104 7 0.1672358469955313 1104 8 0.22728589052856307 1104 9 0.20815073870452494 1104 10 0.37225918670394265 1104 11 0.2133669857355045 1104 12 0.22035772475679494 1104 13 0.21362384346461738 1104 14 0.17350957312961413 1104 15 0.2710271418617367 1104 16 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-1.1475424984063167 2315 17 -1.1140398124753546 2315 18 -1.147297400918447 2315 19 -1.0927457198109494 2315 20 -0.9418379252299545 2315 21 -0.9925898710099545 2315 22 -0.9743127248811589 2315 23 -0.9004129545923121 2315 24 -1.0675574651232296 2315 25 -1.1081229110333624 2316 1 -1.4021850550783554 2316 2 -1.2844436383217896 2316 3 -1.112298378652131 2316 4 -0.9011876658082087 2316 5 -0.8516932465078781 2316 6 -0.9616425746934709 2316 7 -0.9896459305008563 2316 8 -0.9431289605294566 2316 9 -1.2804464242905809 2316 10 -1.1936757276055374 2316 11 -1.2976430513215362 2316 12 -1.3568205788750447 2316 13 -1.2975679484324871 2316 14 -1.5299460577400765 2316 15 -1.4738214565504606 2316 16 -1.5913912235263226 2316 17 -1.901429973305504 2316 18 -1.7843404938881733 2316 19 -1.7650725797964313 2316 20 -1.7346224449060443 2316 21 -1.873363725418713 2316 22 -1.8846534689878993 2316 23 -2.021687637630869 2316 24 -2.059274156737885 2316 25 -1.958904130258702 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13889.0 3 0.0 4 287.0 5 2228.0 6 3039.0 7 2380.0 8 1885.0 9 1680.0 10 1604.0 11 1522.0 12 1430.0 13 1386.0 14 1311.0 15 1436.0 16 1494.0 17 1496.0 18 1626.0 19 2002.0 20 2484.0 21 3193.0 22 4027.0 23 5080.0 24 6302.0 25 7531.0 26 8736.0 27 9896.0 28 11651.0 29 14149.0 30 17829.0 31 23668.0 32 31044.0 33 41447.0 34 56208.0 35 77785.0 36 117164.0 37 222433.0 38 1115682.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.725357423201416 19.321028214603857 13.537321067903793 24.41629329429093 2 14.959478016307862 20.46589961555714 41.556374403865895 23.018247964269104 3 18.84796007491457 25.03727291589502 30.123065289380367 25.991701719810038 4 12.563325989674116 17.30320580169258 38.049254067310336 32.08421414132297 5 12.88446152795012 38.21792783639213 35.583808480579386 13.313802155078363 6 31.45471311193383 37.336930043077935 18.018194580162127 13.190162264826105 7 27.745764018099944 31.874511444582676 22.12289026632463 18.256834270992744 8 24.820465056879797 37.32471741755925 20.536782782260417 17.318034743300537 9 26.513993706190526 14.90122154517647 20.497713043688904 38.087071704944094 10 15.63197775047234 28.680398659391088 34.18178374974126 21.505839840395314 11 33.75814868665826 22.227409536571578 25.33763268712732 18.67680908964285 12 23.97126676293593 24.61428525026797 31.701417007949097 19.713030978847005 13 29.315271643723484 22.130817702848578 26.00951563617307 22.544395017254867 14 20.58041321431599 23.180697878448637 27.81248933610292 28.42639957113246 15 22.655535337456946 31.915057566540845 23.693593431282867 21.735813664719345 16 21.453357468633374 28.16257094881119 28.623996204689213 21.76007537786623 17 21.147035465661197 29.018209716361664 28.305660383588965 21.529094434388167 18 20.997088591571774 26.765144552254 31.415307734219784 20.82245912195444 19 23.21156144950699 26.589154454255464 28.61672291310311 21.582561183134434 20 23.56973347334403 26.733457934049675 29.20010082531464 20.496707767291653 21 22.570555861309852 26.471656576774905 28.182333516785913 22.775454045129333 22 23.16145309324991 28.16843192120309 28.119668186451385 20.550446799095614 23 21.670813650980776 27.797178069360033 29.164774794260595 21.3672334853986 24 23.0585623366817 26.362847852839067 29.626957342966776 20.951632467512454 25 22.16445313273939 27.38882247920203 29.32585729035269 21.120867097705894 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 289.0 1 289.0 2 536.0 3 783.0 4 783.0 5 783.0 6 2028.5 7 3274.0 8 3274.0 9 3274.0 10 4067.5 11 4861.0 12 4861.0 13 4861.0 14 6509.0 15 8157.0 16 8157.0 17 8157.0 18 15000.0 19 21843.0 20 21843.0 21 21843.0 22 35045.5 23 48248.0 24 48248.0 25 48248.0 26 71299.5 27 94351.0 28 94351.0 29 94351.0 30 121206.5 31 148062.0 32 148062.0 33 148062.0 34 164524.0 35 180986.0 36 180986.0 37 180986.0 38 190477.5 39 199969.0 40 199969.0 41 199969.0 42 209954.5 43 219940.0 44 219940.0 45 219940.0 46 230060.5 47 240181.0 48 240181.0 49 240181.0 50 229300.0 51 218419.0 52 218419.0 53 218419.0 54 192011.5 55 165604.0 56 165604.0 57 165604.0 58 141004.0 59 116404.0 60 116404.0 61 116404.0 62 96508.5 63 76613.0 64 76613.0 65 76613.0 66 58294.5 67 39976.0 68 39976.0 69 39976.0 70 29222.0 71 18468.0 72 18468.0 73 18468.0 74 12746.0 75 7024.0 76 7024.0 77 7024.0 78 4767.0 79 2510.0 80 2510.0 81 2510.0 82 1647.5 83 785.0 84 785.0 85 785.0 86 493.0 87 201.0 88 201.0 89 201.0 90 126.0 91 51.0 92 51.0 93 51.0 94 27.5 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16004367629350294 2 0.004898173036493041 3 5.503565209542742E-5 4 9.356060856222661E-4 5 0.11711586765906955 6 0.029113859958481102 7 0.023335116488461226 8 0.020143048666926434 9 0.03390196169078329 10 0.02839839648124055 11 0.03340664082192444 12 0.031425357346489054 13 0.02283979561960238 14 0.006934492164023855 15 0.008255347814314112 16 0.0013758913023856855 17 3.852495646679919E-4 18 7.704991293359838E-4 19 0.0 20 0.0 21 2.2014260838170967E-4 22 0.0044028521676341935 23 0.0011557486940039757 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1817004.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.32275161774183 #Duplication Level Percentage of deduplicated Percentage of total 1 80.62530120726551 51.860412236605605 2 13.027756903527566 16.759623428838502 3 3.414938086770162 6.5897464303585105 4 1.1936569182780807 3.071171898848006 5 0.5707791796096883 1.8357043699306217 6 0.2965496002441843 1.144493176730838 7 0.1780127346621503 0.8015188241527934 8 0.13126154551823524 0.6754483031464287 9 0.0869658958243397 0.5034497144691074 >10 0.4038553136662 4.652135800490531 >50 0.03355762879549305 1.5584898330106658 >100 0.030525774301430147 4.036320924996243 >500 0.004018036778054185 1.9042353127452343 >1k 0.0025647043264175654 3.2550551590470813 >5k 1.7098028842783768E-4 0.7698545836851564 >10k+ 8.549014421391884E-5 0.5823400029447005 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 10590 0.5828275556905763 No Hit GGTATCAACGCAGAGTACTTTTTTT 7085 0.38992759509610325 No Hit TATCAACGCAGAGTACTTTTTTTTT 6915 0.3805715342398806 No Hit CTGTAGGACGTGGAATATGGCAAGA 4990 0.2746279039561828 No Hit GTCCTACAGTGGACATTTCTAAATT 4757 0.2618045970179482 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4415 0.24298240400131205 No Hit CTTTAGGACGTGAAATATGGCGAGG 4146 0.22817781358764208 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3273 0.18013168930833393 No Hit GTACTGGTTCACTATCGGTCAGTCA 2682 0.14760561891993634 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2523 0.1388549502367634 No Hit GTCCTACAGTGTGCATTTCTCATTT 2177 0.11981261461174549 No Hit CTGAAGGACCTGGAATATGGCGAGA 2167 0.11926225809079122 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2016 0.11095187462438168 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.503565209542742E-5 0.0 10 0.0 0.0 0.0 5.503565209542742E-5 0.0 11 0.0 0.0 0.0 1.1007130419085483E-4 0.0 12 0.0 0.0 0.0 1.1007130419085483E-4 0.0 13 0.0 0.0 0.0 1.1007130419085483E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1670 0.0 14.2455845 1 TAGGACC 625 0.0 13.689899 4 ATTGCGA 55 0.0030800288 12.085008 17 GGCGAGG 1100 0.0 11.480758 19 GTTATAC 135 4.6748028E-9 11.267406 3 AATGCCG 135 4.7657522E-9 11.253763 18 ATTTCCG 110 5.000802E-7 11.221793 14 GTAGGAC 2175 0.0 11.14599 3 GGACGTA 60 0.0058832183 11.081583 6 TATTGCG 60 0.005897699 11.077924 16 CGCCCTA 335 0.0 11.054303 16 TGTAGGA 2245 0.0 11.052232 2 TGTCGAG 140 8.416464E-9 10.851844 18 AGGACGT 2680 0.0 10.784215 5 AGGACCG 115 8.732877E-7 10.747198 5 TCCCGCC 355 0.0 10.7007675 13 CCCGTTT 80 3.7806877E-4 10.684635 12 GACGTGG 1245 0.0 10.681044 7 CTGTAGG 2265 0.0 10.587394 1 GCGATTT 135 5.705442E-8 10.553308 11 >>END_MODULE