FastQCFastQC Report
Thu 26 May 2016
SRR1512359_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512359_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280506
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68250.2992756870624326No Hit
GTCCTACAGTGGACATTTCTAAATT62890.27577213127262107No Hit
CTGTAGGACGTGGAATATGGCAAGA59100.2591530125331834No Hit
GTCCTAAAGTGTGTATTTCTCATTT55500.24336704222659356No Hit
CTTTAGGACGTGAAATATGGCGAGG44820.19653533031704368No Hit
GGTATCAACGCAGAGTACTTTTTTT43110.18903699442141347No Hit
TATCAACGCAGAGTACTTTTTTTTT39080.1713654776615365No Hit
GTCCTACAGTGTGCATTTCTCATTT34300.15040521708778667No Hit
CTGTAGGACCTGGAATATGGCGAGA26100.11444828472277643No Hit
CTGAAGGACCTGGAATATGGCGAGA24850.1089670450329883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGCG801.3162935E-714.225857
AAGACCG551.9899328E-413.7941545
TAGGACC11100.013.3275484
GGTATCA13800.013.1075311
TCCAACG1457.2759576E-1212.41130318
ACTGCGG803.0195677E-511.8152668
GGCGAGG14100.011.69064419
CCCGTAC1406.9485395E-1011.52099116
CGGACAT752.1183677E-411.37440910
GTGTAGG4750.011.2369641
CACCTTT8200.011.22445314
CCGTACA1451.2441888E-911.12420717
TTCGCAT600.005931243811.06967617
TGTAGGA35600.011.0549382
CGCCCTA2150.011.03241316
CCACCTT7850.010.874713
AGGACGT33550.010.7696165
CCCCGTA1502.1773303E-910.75387615
CTGTAGG32550.010.7498181
CCCGTTT1502.2064341E-910.74486512