FastQCFastQC Report
Thu 26 May 2016
SRR1512359_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512359_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280506
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78600.3446603516938785No Hit
CTGTAGGACGTGGAATATGGCAAGA68690.30120508343323804No Hit
GTCCTACAGTGGACATTTCTAAATT65450.28699771015730724No Hit
GTCCTAAAGTGTGTATTTCTCATTT55990.2455156881849905No Hit
GGTATCAACGCAGAGTACTTTTTTT54330.2382366018769519No Hit
CTTTAGGACGTGAAATATGGCGAGG54100.23722805377403086No Hit
TATCAACGCAGAGTACTTTTTTTTT52750.23130831490905968No Hit
GTCCTACAGTGTGCATTTCTCATTT37120.1627708938279487No Hit
CTGTAGGACCTGGAATATGGCGAGA30760.13488234628630663No Hit
CTGAAGGACCTGGAATATGGCGAGA29980.13146205271987885No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26500.11620228142350865No Hit
ATTTAGAAATGTCCACTGTAGGACG22990.10081096037458354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.014.1773631
TAGGACC12700.013.1754564
CGAAATC2150.012.36800913
TAAACGT550.003051619512.1001864
AGGACCT23650.011.7386455
GGACCTG21950.011.5985666
TGTAGGA35150.011.4677492
GGCGAGG13950.011.43407319
CGCGCCT1354.7512003E-911.25628412
TGGTATC4450.011.1091542
CTAGGAC4550.011.0744263
CTGTAGG35500.010.9360531
ACGAAAT2350.010.91200812
TCTAGGA4900.010.8649972
GTAGGAC35650.010.7740393
CCAACGA1158.8891466E-710.7327619
AATGTCC8200.010.6575898
AGGACGT35100.010.5909745
GGACGTA909.5229705E-510.555086
TCGCGCG1355.6981662E-810.5546189
Produced by FastQC (version 0.11.5)