FastQCFastQC Report
Thu 26 May 2016
SRR1512358_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512358_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1667466
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66610.39946841494819085No Hit
GGTATCAACGCAGAGTACTTTTTTT42640.25571735795512474No Hit
TATCAACGCAGAGTACTTTTTTTTT39130.2346674534893065No Hit
GTCCTACAGTGGACATTTCTAAATT22990.13787387568921947No Hit
GTCCTAAAGTGTGTATTTCTCATTT21470.12875824754447768No Hit
CTGTAGGACGTGGAATATGGCAAGA20910.12539985822799385No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18950.1136454956203005No Hit
CTTTAGGACGTGAAATATGGCGAGG18880.11322569695574002No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17020.10207104672599022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11800.014.9934981
CCCCGAT400.005336809414.2267457
TTCCGAA655.513651E-513.13832416
TAGGACC3450.012.6456154
GTCTTAG1950.012.1649011
AAGGCGT1052.779434E-711.7423556
GGCGAGG7350.011.72820719
TAGGACA5350.011.5228734
GTACTAG1301.8204446E-811.4045931
GCGATTT752.1174958E-411.37453511
TTAGGAC9200.011.2367273
AGCCGTG600.00594779811.0652467
GTGTAGA3350.010.9166361
GACGTGA8100.010.8896077
CGTGCGC700.001521105210.83158410
TCCGAAT803.814449E-410.67424417
CTAAGAC1252.2916538E-710.6223153
TCCTATA1803.6379788E-1110.53801252
GTAAGAC1909.094947E-1210.4825483
AGGACGT14750.010.4808575
Produced by FastQC (version 0.11.5)