FastQCFastQC Report
Thu 26 May 2016
SRR1512358_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512358_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1667466
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81600.4893653004019272No Hit
GGTATCAACGCAGAGTACTTTTTTT58250.34933246015211106No Hit
TATCAACGCAGAGTACTTTTTTTTT54480.32672330350363965No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26050.1562250744542917No Hit
GTCCTACAGTGGACATTTCTAAATT25010.14998806572367893No Hit
CTGTAGGACGTGGAATATGGCAAGA23920.14345120080409435No Hit
GTCCTAAAGTGTGTATTTCTCATTT22650.13583485360421144No Hit
CTTTAGGACGTGAAATATGGCGAGG22200.13313614790346548No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19810.11880302207061494No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17920.10746845812748206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14100.014.7789351
TAGGACC4200.012.9026374
GGCGAGG7050.011.98688919
AGAACCG657.969059E-411.70187955
GATATAC1751.8189894E-1211.4182461
CTTATAC1701.0913936E-1111.1849483
TTAGTAC951.3488632E-511.0083443
TGGCGAG14600.010.991055518
GTCCTAC13200.010.8848181
TTAGGAC10000.010.8382143
GTCTAAT803.7156598E-410.7046051
GGACCTG8700.010.5904496
ATGGCGA15850.010.36388117
ATATGGC26850.010.36196115
TATGGCG15800.010.33658116
CTGTAGG14700.010.2919571
AGGACGT15550.010.2720685
AGGACAT5000.010.2683995
GTAGGAC13550.010.2439273
GACGTGA8850.010.1959867
Produced by FastQC (version 0.11.5)